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FITC Mouse Anti-Human Cytokeratin
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Cytokeratin-7/-8; CK-7/CK-8;
Mouse BALB/c IgG2a
Human HT29 colorectal carcinoma cell line
Intracellular staining (flow cytometry)
25 μg/mL
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation and Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347653 Rev. 1
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The Anti-Cytokeratin antibody, clone CAM 5.2, is derived from the hybridization of P3/NS-1/1-Ag4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with the human colorectal carcinoma cell line HT29.

The Anti-Cytokeratin antibody has a primary reactivity with human cytokeratin proteins that correspond to Moll’s peptide #7 (cytokeratin 7) and Moll’s peptide #8 (cytokeratin 8), 54 and 53 kilodaltons (kDa), respectively.

347653 Rev. 1
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
347653 Rev.1
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View product citations for antibody "347653" on CiteAb

Development References (8)

  1. Battifora H. Diagnostic uses of antibodies to keratins: A review and immunohistochemical comparison of seven monoclonal and three polyclonal antibodies. In: Fenoglio-Preiser C, Wolff M, Rilke F, ed. Progress in Surgical Pathology. 1988:1-15.
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Cooper D, Schermer A, Sun TT. Biology of disease classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. Lab Invest. 1985; 52:243-256. (Biology). View Reference
  5. Johnson A, Cavallin-Stahl E, Akerman M. Flow cytometric light chain analysis of peripheral blood lymphocytes in patients with non-Hodgkin's lymphoma. Br J Cancer. 1985; 52(2):159-168. (Biology). View Reference
  6. Makin C, Bobrow L, Bodmer W. Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol. 1984; 37(9):975-983. (Biology). View Reference
  7. Moll R, Franke W, Schiller D, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors, and cultured cells. Cell. 1982; 31:11-24. (Biology). View Reference
  8. Smedts F, Ramaekers F, Robben H, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990; 136(3):657-668. (Biology). View Reference
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347653 Rev. 1

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