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FITC Mouse Anti-Human CD42a
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GPIX; GP9; Glycoprotein IX
Mouse BALB/c IgG1, κ
Normal Human Platelets
Flow cytometry
12.5 μg/mL
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation and Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

348083 Rev. 1
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The CD42a antibody, clone Beb1, is derived from hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with normal human platelets.

The CD42a antibody recognizes a 17 to 22-kilodalton (kDa) single-chain, platelet membrane glycoprotein, also known as gpIX that forms a noncovalent complex with gpIb and gpV. Glycoprotein Ib consists of an α–chain disulfide-linked to a β chain. Glycoproteins Ibα (140 to 145 kDa), Ibβ (24 to 25 kDa), and V (82 to 86 kDa) have been designated as CD42b, CD42c and CD42d respectively. CD42a has been reported to react with both gpIX and the gpIb-IX-V complex.

348083 Rev. 1
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
348083 Rev.1
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View product citations for antibody "348083" on CiteAb

Development References (15)

  1. Nieuwenhuis HK. Report on functional studies. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1002-1003.
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Du X, Beutler L, Ruan C, Castaldi PA, Berndt MC. Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane.. Blood. 1987; 69(5):1524-7. (Biology). View Reference
  5. Fox JE, Aggerbeck LP, Berndt MC. Structure of the glycoprotein Ib.IX complex from platelet membranes. J Biol Chem. 1988; 263(10):4882-4890. (Biology). View Reference
  6. Hickey MJ, Williams SA, Roth GJ. Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures. Proc Natl Acad Sci U S A. 1989; 86(17):6773-6777. (Biology). View Reference
  7. Hourdillé P, Heilmann E, Combrié R, Winckler J, Clemetson KJ, Nurden AT. Thrombin induces a rapid redistribution of glycoprotein Ib-IX complexes within the membrane systems of activated human platelets.. Blood. 1990; 76(8):1503-13. (Biology). View Reference
  8. Michelson AD, Benoit SE, Furman MI, Barnard MR, Nurden P, Nurden AT. The platelet surface expression of glycoprotein V is regulated by two independent mechanisms: proteolysis and a reversible cytoskeletal-mediated redistribution to the surface-connected canalicular system.. Blood. 1996; 87(4):1396-408. (Biology). View Reference
  9. Michelson AD, Benoit SE, Kroll MH, et al. The activation-induced decrease in the platelet surface expression of the glycoprotein Ib-IX complex is reversible.. Blood. 1994; 83(12):3562-73. (Biology). View Reference
  10. Michelson AD, Ellis PA, Barnard MR, Matic GB, Viles AF, Kestin AS. Downregulation of the platelet surface glycoprotein Ib-IX complex in whole blood stimulated by thrombin, adenosine diphosphate, or an in vivo wound.. Blood. 1991; 77(4):770-9. (Biology). View Reference
  11. Modderman PW, Admiraal LG, Sonnenberg A, von dem Borne AE. Glycoproteins V and Ib-IX form a noncovalent complex in the platelet membrane.. J Biol Chem. 1992; 267(1):364-9. (Biology). View Reference
  12. Ruggeri ZM. The platelet glycoprotein Ib-IX complex.. Prog Hemost Thromb. 1991; 10:35-68. (Biology). View Reference
  13. Silverstein RL. Schlossman S, Boumsell L, Gilks W, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; :995:1197.
  14. Yamamoto N, Greco NJ, Barnard MR, et al. Glycoprotein Ib (GPIb)-dependent and GPIb-independent pathways of thrombin-induced platelet activation.. Blood. 1991; 77(8):1740-8. (Biology). View Reference
  15. von dem Borne AEGKr, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1000-1002.
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348083 Rev. 1

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