Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version
See More
製品詳細
BD™
L3T4; T-cell surface antigen T4/Leu-3; W3/25; CD4 antigen (p55)
Human
Mouse BALB/c IgG1
Human Peripheral Blood Lymphocytes
Flow cytometry
20 μL
AB_400029
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)
Preparation and Storage
The FITC and PE conjugates are each supplied in 1.0 mL of phosphate-buffered saline containing gelatin and 0.1% sodium azide. Each lot is bottled at titer as determined by immunofluorescence staining of lymphocytes in peripheral blood. You may wish to titrate the reagent for use in your specific application. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
340422 Rev. 1
抗体の詳細
L120
CD4 v4, clone L120, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with human peripheral blood lymphocytes. CD4 recognizes an antigen, approximately 59 kdaltons (kDa), that is present on the helper-inducer subset of T lymphocytes and monocytes. The CD4 antigen is involved in the class II recognition of foreign antigens and has been identified as a receptor for human immunodeficiency virus (HIV). Clone L120 recognizes amino acids 306-370 of the antigen and does not block the binding of the gp120 of HIV, but inhibits the proliferation of CD4 T lymphocytes to class II antigens.
340422 Rev. 1
フォーマットの詳細
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
340422 Rev.1
引用＆参考文献
View product citations for antibody "340422" on CiteAb
Development References (13)
-
Dalgleish A, Beverly P, Clapham P, Crawford D, Greaves M, Weiss R. The CD4 (T4) antigen is an essential component of the receptor for the AIDS virus. Nature. 1984; 312:763-767. (Biology).
-
Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
-
Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to the TH2 antigen. Proc Natl Acad Sci USA. 1981; 78:544-548. (Biology).
-
Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
-
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
-
Lewis DE, Puck JM, Babcock GF, Rich RR. Disproportionate expansion of a minor T cell subset in patients with lymphadenopathy syndrome and acquired immunodeficiency syndrome.. J Infect Dis. 1985; 151(3):555-9. (Biology). View Reference
-
Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.. Cell. 1986; 47(3):333-48. (Biology). View Reference
-
Maino VC, Buck DW, Dalgleish AG. HIV vaccine potential of Anti-CD4 idiotypes. AIDS Research Review. 1990; 1:417-432. (Biology).
-
Merkenschlager M, Buck D, Beverley PCL, Sattenau QJ. Functional epitope analysis of the human CD4 molecule: The MHC class II-dependent activation of resting T cells is inhibited by monoclonal antibodies to CD4 regardless whether or not they recognize epitopes involved in the binding of MHC class II or HIV gp120. J Immunol. 1990; 145(9):2839-2845. (Biology).
-
Ohno T, Kanoh T, Suzuki T, et al. Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus (HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry.. Tohoku J Exp Med. 1988; 154(2):157-72. (Biology). View Reference
-
Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
-
Sattentau QJ, Dalgleish AG, Weiss RA, Beverley PC. Epitopes of the CD4 antigen and HIV infection. Science. 1986; 234(4780):1120-1123. (Biology). View Reference
-
Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence.. Clin Immunol Immunopathol. 1986; 38(2):161-77. (Biology). View Reference
340422 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.