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FITC Mouse Anti-Human CD11a
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LFA-1α; Lymphocyte (Leukocyte) function-associated antigen 1 α chain; ITGAL
Mouse IgG2a
Flow cytometry
6.25 μg/mL
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation and Storage

The monoclonal antibody is supplied as 25 µg purified immunoglobulin in 2.0 mL (12.5 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 12.5 µg in 2.0 mL (6.25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347983 Rev. 1
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CD11a, clone G-25.2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with human cytotoxic T lymphocytes.

CD11a (Anti-LFA-1α) recognizes the Mr 180-kDalton (kDa) a chain of the leucocyte function–associated antigen-1 (LFA-1). LFA-1, a member of a subfamily of integrin receptors, is a leucocyte adhesion receptor that binds to an intercellular adhesion molecule on target tissues. This subfamily consists of three heterodimeric proteins that share a common noncovalently associated β chain (CD18 antigen, Mr 95 kDa) but possess distinct α subunits recognized by CD11a (Anti-LFA-1α), CD11b (Leu-15), and CD11c (Leu-M5).

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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
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Development References (7)

  1. Anderson DC, Springer TA. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins.. Annu Rev Med. 1987; 38:175-94. (Biology). View Reference
  2. Clayberger C, Wright A, Medeiros LJ, et al. Absence of cell surface LFA-1 as a mechanism of escape from immunosurveillance.. Lancet. 1987; 2(8558):533-6. (Biology). View Reference
  3. Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.. Nature. 1989; 341(6243):619-24. (Biology). View Reference
  4. Fischer A, Griscelli C, Blanche S, et al. Prevention of graft failure by an anti-HLFA-1 monoclonal antibody in HLA-mismatched bonemarrow transplantation. Lancet. 1986; 1058-1061. (Biology).
  5. Inghirami G, Wieczorek R, Zhu BY, Silber R, Dalla-Favera R, Knowles DM. Differential expression of LFA-1 molecules in non-Hodgkin's lymphoma and lymphoid leukemia. Blood. 1988; 72(4):1431-1434. (Biology). View Reference
  6. Mentzer SJ, Faller DV, Burakoff SJ. Interferon-gamma induction of LFA-1-mediated homotypic adhesion of human monocytes.. J Immunol. 1986; 137(1):108-13. (Biology). View Reference
  7. Uciechowski P, Schmidt RE. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:543.
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347983 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures. 


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