Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
The CD64 antibody, clone MD22, is derived from the hybridization of P3-X63-Ag8.653 mouse myeloma cells with spleen cells isolated from BALB/c nu/nu mice immunized with FcγRI. The CD64 antibody recognizes a 72-kilodalton (kDa) type I transmembrane glycoprotein that is a high affinity receptor for human IgG. The CD64 antigen is also know as Fc-gamma receptor I or FcγRI.
Development References (14)
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Ernst LK, Duchemin AM, Anderson CL. Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor.. Proc Natl Acad Sci USA. 1993; 90(13):6023-7. (Biology). View Reference
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Fanger NA, Wardwell K, Shen L, Tedder TF, Guyre PM. Type I (CD64) and type II (CD32) Fc gamma receptor-mediated phagocytosis by human blood dendritic cells.. J Immunol. 1996; 157(2):541-8. (Biology). View Reference
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Fischer G, Schneider EM, L Moldawer LL, et al. CD64 surface expression on neutrophils is transiently upregulated in patients with septic shock.. Intensive Care Med. 2001; 27(12):1848-52. (Biology). View Reference
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Qureshi SS, Lewis SM, Gant VA, Treacher D, Davis BH, Brown KA. Increased distribution and expression of CD64 on blood polymorphonuclear cells from patients with the systemic inflammatory response syndrome (SIRS).. Clin Exp Immunol. 2001; 125(2):258-65. (Biology). View Reference
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Scholl PR, Geha RS. Physical association between the high-affinity IgG receptor (FcγRI) and the γ subunit of the high-affinity IgE receptor (FcεRIγ). Proc Natl Acad Sci USA. 1993; 90:8847-8850. (Biology).
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Valerius T, Repp R, de Wit TP, et al. Involvement of the high-affinity receptor for IgG (Fc γ RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy. Blood. 1993; 82:931-939. (Biology).
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Wang AV, Scholl PR, Geha RS. Physical and functional association of the high affinity immunoglobulin G receptor (Fc gamma RI) with the kinases Hck and Lyn.. J Exp Med. 1994; 180(3):1165-70. (Biology). View Reference
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van de Winkel JG, Anderson CL. Biology of human immunoglobulin G Fc receptors.. J Leukoc Biol. 1991; 49(5):511-24. (Biology). View Reference
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Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.