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      Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5

      BD Quantibrite™ Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5

      (RUO (GMP))
      製品詳細
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      BD Quantibrite™
      Human
      Flow cytometry
      RUO (GMP)
      Buffered gelatin solution with an HLA-DR stabilizing reagent and 0.1% sodium azide.


      説明

      Anti–HLA-DR, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

      CD14, clone MφP9, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis.

      Anti–HLA-DR recognizes a human class II major histocompatibility complex (MHC) antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDaltons (kDa), respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules.

      Anti-Monocyte PerCP-Cy5.5 reagent recognizes monocytes using two discrete specificities. This reagent includes CD14, a human monocyte/macrophage antigen, Mr 55 kDa, that recognizes the majority of the monocyte population. In addition, Anti-Monocyte PerCP-Cy5.5 recognizes FcR1 (CD64) by taking advantage of the unique recognition of CD64 by cyanine dyes. Anti-Monocyte PerCP-Cy5.5 is specially formulated to take advantage of this property of the cyanine dye to ensure detection of all monocytes (CD14 brightly positive and weakly positive) in a sample.

      調製と保管

      The Anti–HLA-DR/Anti-Monocyte PerCP-Cy5.5 reagent is supplied as a combination of Anti–HLA-DR PE (≥95% 1:1 PE:mAb ratio) and Anti-Monocyte PerCP-Cy5.5 in a buffered gelatin solution containing an HLA-DR stabilizing reagent and 0.1% sodium azide. Store vials in the dark at 2° to 8°C. Do not freeze QuantiBRITE reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340827 Rev. 1
      構成品
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      説明 クローン アイソタイプ EntrezGene ID
      HLA-DR 1 1 RPE N/A N/A N/A
      CD14 PerCP-Cy5.5 N/A N/A N/A
      340827 Rev. 1
      引用＆参考文献
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      開発者向け参考資料 (22)

      1. Asadullah K, Woiciechowsky C, Docke WD, et al. Immunodepression following neurosurgical procedures. Crit Care Med. 1995; 23:1976-1983. (Biology).
      2. Bernstein ID, Self S. Joint report of the Myeloid Section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986:1-25.
      3. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.. Immunogenetics. 1984; 19(3):179-94. (Biology). 参考文献を見る
      4. Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983; 130(1):145-152. (Biology). 参考文献を見る
      5. Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression.. J Immunol. 1986; 137(2):490-7. (Biology). 参考文献を見る
      6. Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.. Proc Natl Acad Sci USA. 1981; 78(3):1791-5. (Biology). 参考文献を見る
      7. Goyert SM, Ferrero E. Biochemical analysis of myeloid antigens and cDNA expression of gp55 (CD14). In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:613-619.
      8. Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia, I: Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983; 47:157-163. (Biology).
      9. Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:796-797.
      10. Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line.. J Immunol. 1980; 125(1):293-9. (Biology). 参考文献を見る
      11. Levacher M, Tallet S, Dazza MC, Dournon E, Rouveix B, Pocidalo JJ. T activation marker evaluation in ARC patients treated with AZT. Comparison with CD4+ lymphocyte count in non-progressors and progressors towards AIDS.. Clin Exp Immunol. 1990; 81(2):177-82. (Biology). 参考文献を見る
      12. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Biology). 参考文献を見る
      13. Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence.. Clin Immunol Immunopathol. 1986; 38(2):161-77. (Biology). 参考文献を見る
      14. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). 参考文献を見る
      15. Tomkinson BE, Wagner DK, Nelson DL, Sullivan JL. Activated lymphocytes during acute Epstein-Barr virus infection.. J Immunol. 1987; 139(11):3802-7. (Biology). 参考文献を見る
      16. Van Den Berk JMM, Oldenburger RHJ, Van Den Berg AP, et al. Low HLA-DR expression on monocytes as a prognostic marker for bacterial sepsis after liver transplantation. Trans. 1997; 63:1846-1848. (Biology).
      17. Wakefield CH, Carey PD, Foulds S, Monson JRT, Guillou PJ. Changes in major histocompatibility complex class II expression in monocytes and T cells of patients developing infection after surgery. Brit J Surg. 1993; 80:205-209. (Biology).
      18. Warnke R, Miller R, Grogan T, Pederson M, Dilley J, Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma.. N Engl J Med. 1980; 303(6):293-300. (Biology). 参考文献を見る
      19. Warnke RA, Levy R. Detection of T and B cell antigens with hybridoma monoclonal antibodies: a biotinavidin-horseradish peroxidase method. J Histochem Cytochem. 1980; 28:771-776. (Biology).
      20. Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). 参考文献を見る
      21. Zipf TF, Fox RI, Dilley J, Levy R. Definition of the high-risk acute lymphoblastic leukemia patient by immunological phenotyping with monoclonal antibodies.. Cancer Res. 1981; 41(11 Pt 2):4786-9. (Biology). 参考文献を見る
      22. van Vugt MK, van den Herik-Oudijk IE, van de Winkel JGJ. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996; 88:2358-2360. (Biology).
      すべて表示する (22) 表示項目を減らす
      340827 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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