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Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5

BD Quantibrite™ Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5

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BD Quantibrite™
Flow cytometry
Buffered gelatin solution with an HLA-DR stabilizing reagent and 0.1% sodium azide.


Anti–HLA-DR, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

CD14, clone MφP9, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis.

Anti–HLA-DR recognizes a human class II major histocompatibility complex (MHC) antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDaltons (kDa), respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules.

Anti-Monocyte PerCP-Cy5.5 reagent recognizes monocytes using two discrete specificities. This reagent includes CD14, a human monocyte/macrophage antigen, Mr 55 kDa, that recognizes the majority of the monocyte population. In addition, Anti-Monocyte PerCP-Cy5.5 recognizes FcR1 (CD64) by taking advantage of the unique recognition of CD64 by cyanine dyes. Anti-Monocyte PerCP-Cy5.5 is specially formulated to take advantage of this property of the cyanine dye to ensure detection of all monocytes (CD14 brightly positive and weakly positive) in a sample.


The Anti–HLA-DR/Anti-Monocyte PerCP-Cy5.5 reagent is supplied as a combination of Anti–HLA-DR PE (≥95% 1:1 PE:mAb ratio) and Anti-Monocyte PerCP-Cy5.5 in a buffered gelatin solution containing an HLA-DR stabilizing reagent and 0.1% sodium azide. Store vials in the dark at 2° to 8°C. Do not freeze QuantiBRITE reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340827 Rev. 1
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説明 クローン アイソタイプ EntrezGene ID
CD14 PerCP-Cy5.5 N/A N/A N/A
340827 Rev. 1
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開発者向け参考資料 (22)

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  7. Goyert SM, Ferrero E. Biochemical analysis of myeloid antigens and cDNA expression of gp55 (CD14). In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:613-619.
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  9. Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:796-797.
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  12. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Biology). 参考文献を見る
  13. Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence.. Clin Immunol Immunopathol. 1986; 38(2):161-77. (Biology). 参考文献を見る
  14. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). 参考文献を見る
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340827 Rev. 1

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