Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      CD4 FITC/CD8 PE
      製品詳細
      Down Arrow Up Arrow


      BD Simultest™
      Human
      Flow cytometry
      体外診断用医薬品
      Buffered saline with gelatin and 0.1% sodium azide.


      説明

      BD Simultest™ CD4/CD8 is a two-color direct immunofluorescence reagent for enumerating percentages of mature human helper/inducer (CD4+) and suppressor/cytotoxic (CD8+) lymphocytes in erythrocyte-lysed whole blood (LWB). The helper/suppressor ratio (CD4+/CD8+) can also be determined.

      調製と保管

      • For in vitro diagnostic use.

      • When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

      • The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

      • Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

      • The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      340039 Rev. 1
      構成品
      Down Arrow Up Arrow
      説明 クローン アイソタイプ EntrezGene ID
      CD8 PE SK1 IgG1, κ N/A
      CD4 FITC SK3 IgG1, κ 920
      340039 Rev. 1
      引用＆参考文献
      Down Arrow Up Arrow

      開発者向け参考資料 (26)

      1. A National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A3). 1991. (Biology).
      2. Antel J, Bania M, Noronha A, Neely S. Defective suppressor cell function mediated by T8+ cell lines from patients with progressive multiple sclerosis. J Immunol. 1986; 137:3436-3439. (Biology).
      3. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.
      4. Bishop G, Hall B, Duggin G, Horvath J, Sheil A, Tiller D. Immunopathology of renal allograft rejection analyzed with monoclonal antibodies to mononuclear cell markers. Kidney Internat. 1986; 29:708-717. (Biology).
      5. Centers for Disease Control. Guidelines for the performance of CD4+ T-cell determination in persons with human immunodeficiency virus infection. MMWR. 1992; 41:1-17. (Biology).
      6. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes. NCCLS document H42-T. 1992. (Biology).
      7. Dalgleish A, Beverly P, Clapham P, Crawford D, Greaves M, Weiss R. The CD4 (T4) antigen is an essential component of the receptor for the AIDS virus. Nature. 1984; 312:763-767. (Biology).
      8. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). 参考文献を見る
      9. Gallagher PF, Fazekas de St. Groth B, Miller JFAP. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Proc Natl Acad Sci USA. 1989; 86:10044-10048. (Biology).
      10. Giorgi J, Hultin L. Lymphocyte subset alterations and immunophenotyping by flow cytometry in HIV disease. Clin Immunol Newslett. 1990; 10(4):55-61. (Biology).
      11. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
      12. Gratama J, Naipal A, Oljans P, et al. T lymphocyte repopulation and differentiation after bone marrow transplantation: Early shifts in the ratio between T4+ and T8+ T lymphocytes correlate with the occurrence of acute graft-versus-host disease. Blood. 1984; 63(6):1416-1423. (Biology).
      13. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
      14. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). 参考文献を見る
      15. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). 参考文献を見る
      16. Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.. Cell. 1986; 47(3):333-48. (Biology). 参考文献を見る
      17. Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
      18. Ohno T, Kanoh T, Suzuki T, et al. Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus (HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry.. Tohoku J Exp Med. 1988; 154(2):157-72. (Biology). 参考文献を見る
      19. Prince H, Hirji K, Waldbeser L, Plaeger-Marshall S, Kleinman S, Lanier L. Influence of racial background on the distribution of T cell subsets and Leu-11-positive lymphocytes in healthy blood donors. Diag Immunol. 1985; 3:33-37. (Biology).
      20. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). 参考文献を見る
      21. Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
      22. Schmidt RE. Monoclonal antibodies for diagnosis of immunodeficiencies. Blut. 1989; 59:200-206. (Biology).
      23. Smolen J, Chused T, Leiserson W, Reeves J, Alling D, Steinberg A. Heterogeneity of immunoregulatory T-cell subsets in systemic lupus erythematosus. Am J Med. 1982; 72:783-790. (Biology).
      24. Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence.. Clin Immunol Immunopathol. 1986; 38(2):161-77. (Biology). 参考文献を見る
      25. Wolde-Mariam W, Peter JB. Recent diagnostic advances in cellular immunology. Diag Med. 1984; 7:25-32. (Biology).
      26. Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). 参考文献を見る
      すべて表示する (26) 表示項目を減らす
      340039 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.