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      Purified Mouse Anti- ZAP-70 Kinase
      Z24820_610240_image1.png

      Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000 dilution of the mouse anti- ZAP-70 Kinase antibody. Investigators should note that another unidentified band may be observable migrating above the 70 kDa (ZAP-70 Kinase) target.

      Z24820_610240_image1.png Blue-Cap-White-Label.jpg

      Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000 dilution of the mouse anti- ZAP-70 Kinase antibody. Investigators should note that another unidentified band may be observable migrating above the 70 kDa (ZAP-70 Kinase) target.

      商品详情
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      BD Transduction Laboratories™
      Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
      Mouse IgG2a
      Human ZAP-70 Kinase aa. 468-619
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Not Recommended)
      70 kDa
      250 µg/ml
      AB_397635
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      610239 Rev. 2
      抗体详情
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      29/ZAP70 Kinase

      ZAP-70 is a protein tyrosine kinase (PTK) that associates with the ζ subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and ζ subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and ζ. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.

      610239 Rev. 2
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610239 Rev.2
      报价单和参考
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      View product citations for antibody "610239" on CiteAb

      研发参考 (5)

      1. Arpaia E, Shahar M, Dadi H, Cohen A, Roifman CM. Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase. Cell. 1994; 76(5):947-958. (Biology). 查看参考
      2. Chan AC, Kadlecek TA, Elder ME, et al. ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency. Science. 1994; 264:1599-1601. (Biology).
      3. Maccalli C, Pisarra P, Vegetti C, Sensi M, Parmiani G, Anichini A. Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions. J Immunol. 1999; 163(12):6912-6923. (Biology: Flow cytometry). 查看参考
      4. Michel F, Attal-Bonnefoy G, Mangino G, Mise-Omata S, Acuto O. CD28 as a molecular amplifier extending TCR ligation and signaling capabilities. Immunity. 2001; 15(6):935-945. (Biology: Western blot). 查看参考
      5. Mora AL, Stanley S, Armistead W, Chan AC, Boothby M. Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel. J Immunol. 2001; 167(10):5628-5635. (Biology: Western blot). 查看参考
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      610239 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.