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      Purified Mouse Anti-TrkB
      商品详情
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      BD Transduction Laboratories™
      Neurotrophic Tyrosine Kinase Receptor Type 2; NTRK2
      Rat (QC Testing)
      Mouse IgG1
      Human TrkB aa. 156-322
      Western blot (Routinely Tested), Bioimaging, Fluorescence microscopy, Immunohistochemistry, Immunoprecipitation (Not Recommended)
      116-145 & 70-95 kDa
      250 µg/ml
      AB_397508
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      610102 Rev. 5
      抗体详情
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      47/TrkB

      The full-length TrkB gene has been reported to encode for a 145 kDa glycosylated transmembrane tyrosine kinase and neurotrophin receptor. The same gene also has been reported to encode for a 95 kDa glycoprotein that is identical to gp145 [TrkB] at the extracellular domain and transmembrane portion but lacks the intracellular portion.  TrkB has been observable in a range of 116-145 kD and 70-95 kD due to various TrkB maturation states, subcellular localizations, and glycosylation states.  TrkB belongs to a family of tyrosine kinases that include the TrkA proto-oncogene and TrkC. All have an extracellular ligand-binding domain, a transmembrane region, and intracellular kinase and autophosphorylation domains. TrkB binds the neurotrophins NT3 and NT4/5, as well as brain-derived neurotrophic factor (BDNF), a peptide that helps motor neuron survival and repair. The TrkB tyrosine kinase is activated upon binding to BDNF resulting in autophosphorylation of residues Y670, Y674 and Y675 and the subsequent association of several intracellular proteins like PLCγ, Shc, and PI3-Kinase. TrkB is widely expressed in cells of neuroepithelium and neural crest origin. Some of these include motor neurons, dopamine-producing neurons, and neurons which release γ-aminobutyric acid in the substantia nigra, neocortex, and hippocampus. The two TrkB gene products are differentially expressed in regions of the adult brain.

      610102 Rev. 5
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610102 Rev.5
      报价单和参考
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      研发参考 (7)

      1. Armanini MP, McMahon SB, Sutherland J, Shelton DL, Phillips HS. Truncated and catalytic isoforms of trkB are co-expressed in neurons of rat and mouse CNS.. Eur J Neurosci. 1995; 7(6):1403-1409. (Biology).
      2. Du J, Feng L, Yang F, Lu B. Activity- and Ca(2+)-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons. J Cell Biol. 2000; 150(6):1423-1434. (Biology: Western blot). 查看参考
      3. Ebendal T. Function and evolution in the NGF family and its receptors. J Neurosci Res. 1992; 32(4):461-470. (Biology). 查看参考
      4. Jovanovic JN, Czernik AJ, Fienberg AA, Greengard P, Sihra TS. Synapsins as mediators of BDNF-enhanced neurotransmitter release. Nat Neurosci. 1992; 3(4):323-329. (Biology: Western blot). 查看参考
      5. Klein R, Conway D, Parada LF, Barbacid M. trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell. 1990; 61(4):647-656. (Biology). 查看参考
      6. Kryll D, Yacoubian T, Haapasalo A, Castren E, Lo D, Barker P. Subcellular localization of full-length and truncated trk receptor isoforms in polarized neurons and epithelial cells.. J Neurosci. 1999; 19(14):5823-5833. (Biology).
      7. Muller D, Djebbara-Hannas Z, Jourdain P. Brain-derived neurotrophic factor restores long-term potentiation in polysialic acid-neural cell adhesion molecule-deficient hippocampus. Proc Natl Acad Sci U S A. 2000; 97(8):4315-4320. (Biology: Western blot). 查看参考
      查看所有文件 (7) 查看更少内容
      610102 Rev. 5

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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