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      Purified Mouse Anti-RORγt
      Purified Mouse Anti-RORγt
      Western blot analysis of RORγt expression by mouse thymocytes. Cell lysate was prepared from untreated C57BL/6 mouse thymus (lanes 1-3). The lysate was electrophoresed (SDS-PAGE; approximately 12.5 μg of total cellular protein per lane), transferred to a PVDF membrane and then probed with Purified Mouse Anti-RORγt antibody (Clone O28-835; Cat. No. 562197) at concentrations of 1.25 (lane 1), 0.6 (lane 2), and 0.3 µg/ml (lane 3). Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002) and a chemiluminescent detection system. RORgt is identified as a band of ~56 kDa in the thymus cell lysate.
      Purified Mouse Anti-RORγt
      Western blot analysis of RORγt expression by mouse thymocytes. Cell lysate was prepared from untreated C57BL/6 mouse thymus (lanes 1-3). The lysate was electrophoresed (SDS-PAGE; approximately 12.5 μg of total cellular protein per lane), transferred to a PVDF membrane and then probed with Purified Mouse Anti-RORγt antibody (Clone O28-835; Cat. No. 562197) at concentrations of 1.25 (lane 1), 0.6 (lane 2), and 0.3 µg/ml (lane 3). Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002) and a chemiluminescent detection system. RORgt is identified as a band of ~56 kDa in the thymus cell lysate.
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      BD Pharmingen™
      Rorc; Rorg; RORgamma; Rorγ; RORgt; RORgammat; RORγt; TOR; Thor; Nr1f3; ROR
      Mouse (QC Testing), Human (Tested in Development)
      Mouse IgG2b, κ
      Human RORγt Recombinant Protein
      Western blot (Routinely Tested)
      56–58 kDa
      0.5 mg/ml
      AB_10894594
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      562197 Rev. 1
      抗体详情
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      O28-835

      The O28-835 monoclonal antibody recognizes ROR-gamma (RORγ) and its isoform, RORγt. RORγ (~58 kDa calculated molecular weight) is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. It is expressed in different tissues including skeletal muscle, kidney, liver and adipose tissues. RORγt (~56 kDa calculated molecular weight) is a RORγ isoform that is expressed exclusively by lymphoid cells. It is expressed by various lymphoid cell types including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells and lymphoid tissue inducer (Lti) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.

      562197 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      562197 Rev.1
      报价单和参考
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      研发参考 (6)

      1. Eberl G, Littman DR. The role of the nuclear hormone receptor RORgt in the development of lymph nodes and Peyer's patches. Immunol Rev. 2003; 195(195):81-90. (Biology). 查看参考
      2. Eberl G, Marmon S, Sunshine MJ, Rennert PD, Choi Y, Littman DR. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol. 2004; 5(1):64-73. (Biology). 查看参考
      3. Hamada H, Garcia-Hernandez MdlL, Reome JB, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009; 182(6):3469-3481. (Biology). 查看参考
      4. He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells. Immunity. 1998; 9(6):797-806. (Biology). 查看参考
      5. Ivanov, II, McKenzie BS, Zhou L, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006; 126(6):1121-1133. (Biology). 查看参考
      6. Medvedev A, Yan ZH, Hirose T, Giguere V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996; 181(1-2):199-206. (Biology). 查看参考
      查看所有文件 (6) 查看更少内容
      562197 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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