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      Purified Mouse Anti-ROCK-I
      611136_IMAGE1.png

      Western blot analysis for ROCK-I.  A mouse kidney lysate (left) or mouse cerebrum lysate (right) was used with the Mouse Anti-ROCK-I antibody at a 1:500 dilution.  ROCK-I is expected to be observed migrating at ~ 160 kDa.

      611136_IMAGE1.png Blue-Cap-White-Label.jpg

      Western blot analysis for ROCK-I.  A mouse kidney lysate (left) or mouse cerebrum lysate (right) was used with the Mouse Anti-ROCK-I antibody at a 1:500 dilution.  ROCK-I is expected to be observed migrating at ~ 160 kDa.

      商品详情
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      BD Transduction Laboratories™
      ROKβ
      Mouse (QC Testing), Human, Rat (Tested in Development)
      Mouse IgG1
      Mouse ROCK-I aa. 906-1012
      Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
      160 kDa
      250 µg/ml
      AB_398448
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      611137 Rev. 2
      抗体详情
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      46/ROCK-I

      ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers. It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif. The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II. ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD). As a result, the kinase activity of ROCK-I is moderately stimulated. The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain. This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase. In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA. NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton. Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation.

      611137 Rev. 2
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      611137 Rev.2
      报价单和参考
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      View product citations for antibody "611137" on CiteAb

      研发参考 (5)

      1. Ishizaki T, Maekawa M, Fujisawa K, et al. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 1996; 15(8):1885-1893. (Biology). 查看参考
      2. Nakagawa O, Fujisawa K, Ishizaki T, Saito Y, Nakao K, Narumiya S. ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice. FEBS Lett. 1996; 392(2):189-193. (Biology). 查看参考
      3. Pawlak G, Helfman DM. Post-transcriptional down-regulation of ROCKI/Rho-kinase through an MEK-dependent pathway leads to cytoskeleton disruption in Ras-transformed fibroblasts. Mol Biol Cell. 2002; 13(1):336-347. (Biology: Western blot). 查看参考
      4. Sahai E, Olson MF, Marshall CJ. Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility. EMBO J. 2001; 20(4):755-766. (Biology: Western blot). 查看参考
      5. Wang H, Eto M, Steers WD, Somlyo AP, Somlyo AV. RhoA-mediated Ca2+ sensitization in erectile function. J Biol Chem. 2002; 277(37):30614-30621. (Biology: Western blot). 查看参考
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      611137 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.