Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
Products

Link Arrow
解决方案

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu

Dear Customer,  If you experience any text readability or display issues on our website, please update your web browser to the latest version

See More

Close
Website Feedback
Alert Icon
R56220_610651_image1.png

Western blot analysis of Rac1 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Rac1 antibody.

R56220_610651_image2.png

Immunofluorescent staining of human fibroblast cells.

R56220_610651_image1.png R56220_610651_image2.png Blue-Cap-White-Label.jpg

Western blot analysis of Rac1 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Rac1 antibody.

Immunofluorescent staining of human fibroblast cells.

商品详情
Down Arrow Up Arrow


BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
Mouse IgG2b, κ
Human Rac1
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
21 kDa
250 µg/ml
AB_397978
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
显示较多信息 blue down arrow 显示较少信息 blue up arrow
610651 Rev. 1
抗体详情
Down Arrow Up Arrow
102/Rac1

Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, like many of its relatives, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. In addition, IQGAP1 has been identified as a GTPase activating protein specific for Rac1. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. BD Biosciences, however, has not performed such cross-reactivity testing.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610651 Rev. 1
格式详情
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610651 Rev.1
报价单和参考
Down Arrow Up Arrow
View product citations for antibody "610651" on CiteAb

研发参考 (5)

  1. Didsbury J, Weber RF, Bokoch GM, Evans T, Snyderman R. rac, a novel ras-related family of proteins that are botulinum toxin substrates. J Biol Chem. 1989; 264(28):16378-16382. (Biology). 查看参考
  2. Doye A, Mettouchi A, Bossis G, et al. CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion. Cell. 2002; 111(4):553-564. (Clone-specific: Immunofluorescence, Western blot). 查看参考
  3. Habas R, Dawid IB, He X. Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation. Genes Dev. 2003; 17(2):295-309. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
  4. Innocenti M, Tenca P, Frittoli E, et al. Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002; 156(1):125-136. (Clone-specific: Immunofluorescence, Western blot). 查看参考
  5. Nobes CD, Hall A. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell. 1995; 81(1):53-62. (Biology). 查看参考
查看所有文件 (5) 查看更少内容
610651 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.