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Western blot analysis of Intersectin on rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Intersectin antibody.
BD Transduction Laboratories™ Purified Mouse Anti-Intersectin/ESE-1
监管状态图例
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准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
ESE-1 (epithelium-specific, EH domain and SH3 domain regulator of endocytosis) contains two N-terminal EH (Ep15 homology) domains, a central coiled-coil domain, and five C-terminal SH3 domains. ESE-1 is also known as the adaptor protein Intersectin. Intersectin constitutively associates with Eps15 to form a complex that is analogous to the yeast Pan1p-End3p complex, which is required for endocytosis and organization of the actin cytoskeleton. The EH, coiled-coil, and SH3 domains of intersectin bind epsin family proteins, SNAP25 and SNAP23, and dynamin, respectively. Thus, intersectin functions in complex with Eps15 to regulate endocytosis by linking components of the endocytic machinery.
研发参考 (2)
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Okamoto M, Schoch S, Sudhof TC. EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis. J Biol Chem. 1999; 274(26):18446-18454. (Biology). 查看参考
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Sengar AS, Wang W, Bishay J, Cohen S, Egan SE. The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15. EMBO J. 1999; 18(5):1159-1171. (Biology). 查看参考
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