BD Transduction Laboratories™ Purified Mouse Anti-ILK
克隆 3/ILK (RUO)
Western blot analysis of ILK on Hs68 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-ILK antibody.
Western blot analysis of ILK on Hs68 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-ILK antibody.
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of α and β subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. In addition, the interaction of ILK with the cytoplasmic domains of integrin β1 and β3 subunits and localization of ILK to focal adhesion plaques suggests a role for ILK in the regulation of cell adhesion. Interestingly, overexpression of ILK in epithelial cells inhibits cell adhesion and stimulates cell cycle progression and tumorigenicity. Thus, ILK regulates signal transduction in many different cell signaling pathways.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (4)
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Delcommenne M, Tan C, Gray V, Rue L, Woodgett J, Dedhar S. Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc Natl Acad Sci U S A. 1998; 95(19):11211-11216. (Biology). 查看参考
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Hannigan GE, Leung-Hagesteijn C, Fitz-Gibbon L, et al. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature. 1996; 379(6560):91-96. (Biology). 查看参考
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Nikolopoulos SN, Turner CE. Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions. J Biol Chem. 2001; 276(26):23499-23505. (Clone-specific: Immunofluorescence, Western blot). 查看参考
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Persad S, Attwell S, Gray V, et al. Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells. Proc Natl Acad Sci U S A. 2000; 97(7):3207-3712. (Biology). 查看参考
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