BD Pharmingen™ Purified Mouse Anti-Human DNA Topoisomerase I
克隆 C-21 (RUO)
Western blot analysis of DNA Topoisomerase I. Lysate from A-431 human epidermal carcinoma cell was probed with anti-human DNA Topoisomerase I (Cat. No. 556597) at 1.0 (lane 1), 0.2 (lane 2), and 0.04 µg/ml (lane 3). Clone C-21 identifies Topo I as an ~100 kDa band.
Western blot analysis of DNA Topoisomerase I. Lysate from A-431 human epidermal carcinoma cell was probed with anti-human DNA Topoisomerase I (Cat. No. 556597) at 1.0 (lane 1), 0.2 (lane 2), and 0.04 µg/ml (lane 3). Clone C-21 identifies Topo I as an ~100 kDa band.
准备和存储
推荐的实验流程
The C-21 antibody is recommended for western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL-1555) or K562 human leukemia cells (ATCC CCL-243) may be used as a positive control for this application.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during DNA replication. The correction of DNA damage, so called "proofreading" functions of the cell, is achieved by numerous excision-repair enzymes. Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing other excision-repair enzymes to correct DNA errors. Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly constant throughout the cell cycle. The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between cell types and during the cell cycle. In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes. DNA Topo I migrates at a molecular weight of 100 kDa in SDS-PAGE. Clone C-21 recognizes human DNA Topoisomerase I. The antibody is routinely tested by western blot analysis of A-431 cell lysates.
研发参考 (2)
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Sugimoto Y, Tsukahara S, Oh-hara T, Isoe T, Tsuruo T. Decreased expression of DNA topoisomerase I in camptothecin-resistant tumor cell lines as determined by a monoclonal antibody. Cancer Res. 1990; 50(21):6925-6930. (Biology). 查看参考
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Tazi J, Rossi F, Labourier E, Gallouzi I, Brunel C, Antoine E. DNA topoisomerase I: customs officer at the border between DNA and RNA worlds. J Mol Med. 1997; 75(11-12):786-800. (Biology). 查看参考
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