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      Purified Mouse anti-Human CD325
      561553.png

      Western blot analysis of N-Cadherin in H9-derived neural stem cells (NSC) and transformed human epithelioid carcinoma (HeLa).  A NSC lysate (left panel) and a HeLa cell lysate (right panel) (Cat. No.611449) were probed with Purified Mouse anti-Human CD325 monoclonal antibody at concentrations of 0.5, 0.25, and 0.125 µg/ml (lanes 1, 2, and 3, respectively). N-Cadherin is identified as a band of 130 kDa.

      561553.png Blue-Cap-White-Label.jpg

      Western blot analysis of N-Cadherin in H9-derived neural stem cells (NSC) and transformed human epithelioid carcinoma (HeLa).  A NSC lysate (left panel) and a HeLa cell lysate (right panel) (Cat. No.611449) were probed with Purified Mouse anti-Human CD325 monoclonal antibody at concentrations of 0.5, 0.25, and 0.125 µg/ml (lanes 1, 2, and 3, respectively). N-Cadherin is identified as a band of 130 kDa.

      商品详情
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      BD Pharmingen™
      Cadherin-2, N-Cadherin; NCAD; CDHM; CDH2
      Human (QC Testing)
      Mouse IgG1, κ
      Human extracellular N-Cadherin domain Recombinant Protein
      Western blot (Routinely Tested), Bioimaging, Flow cytometry, Immunofluorescence (Tested During Development)
      130 kDa
      0.5 mg/ml
      1000
      AB_10713831
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Because the extracellular domain of N-Cadherin is trypsin-sensitive, it is important to avoid using trypsin to dissociate the cells to be studied.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      561553 Rev. 1
      抗体详情
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      8C11

      The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325). Cadherins are a family of Ca2+ -dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (α, β, γ). Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of β-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates β-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.

      561553 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      561553 Rev.1
      报价单和参考
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      View product citations for antibody "561553" on CiteAb

      研发参考 (3)

      1. Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol. 1995; 130:66-77. (Biology). 查看参考
      2. Puch S, Armeanu S, Kibler C, et al. N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. J Cell Sci. 2001; 114(8):1567-1577. (Clone-specific: Flow cytometry). 查看参考
      3. Wein F, Pietsch L, Saffrich R, et al. N-Cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells. Stem Cell Res. 2010; 4(2):129-139. (Clone-specific: Flow cytometry). 查看参考
      561553 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.