Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Human Caspase-8
      Purified Mouse Anti-Human Caspase-8
      Western blot analysis of caspase-8. Lysates from control (lanes 1-3) and camptothecin treated Jurkat cells (lanes 4-6) were probed with anti-human caspase-8 (clone 4-1-20, Cat. No. 551245) at concentrations of: 4.0 (lane 1), 2.0 (lane 2), and 1.0 µg/ml (lane 3). Caspase-8 is identified as 55/50 kDa (proform) and 40/36 kDa (cleaved) bands in treated cells and the 55 kDa in control cells.
      Purified Mouse Anti-Human Caspase-8
      (+)=positive, (-)=negative, (NT)=not tested
      Purified Mouse Anti-Human Caspase-8 Purified Mouse Anti-Human Caspase-8
      Western blot analysis of caspase-8. Lysates from control (lanes 1-3) and camptothecin treated Jurkat cells (lanes 4-6) were probed with anti-human caspase-8 (clone 4-1-20, Cat. No. 551245) at concentrations of: 4.0 (lane 1), 2.0 (lane 2), and 1.0 µg/ml (lane 3). Caspase-8 is identified as 55/50 kDa (proform) and 40/36 kDa (cleaved) bands in treated cells and the 55 kDa in control cells.
      (+)=positive, (-)=negative, (NT)=not tested
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      FLICE, MACH-1, Mch5
      Human (QC Testing)
      Mouse IgG1, κ
      Human caspase-8 recombinant protein
      Western blot (Routinely Tested)
      55/50 kDa
      0.5 mg/ml
      AB_394117
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Applications include western blot analysis (1 - 2 µg/ml). Jurkat T cells (ATCC CRL-1573) are suggested as positive controls. BD Biosciences Pharmingen offers several caspase-8 antibodies. A Jurkat model cell system was used to evaluate these antibodies; these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      551244 Rev. 4
      抗体详情
      Down Arrow Up Arrow
      4-1-20

      Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50 kDa doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23 kDa. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. The antibody recognizes both the proform of human caspase-8 (55/50 kDa doublet) as well as the cleaved form (40/36 kDa doublet) on SDS/PAGE. Full-length recombinant human caspase-8 protein was used as immunogen.

      551244 Rev. 4
      格式详情
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      551244 Rev.4
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "551244" on CiteAb

      研发参考 (5)

      1. Boesen-de Cock JG, Tepper AD, de Vries E, van Blitterswijk WJ, Borst J. Common regulation of apoptosis signaling induced by CD95 and the DNA-damaging stimuli etoposide and gamma-radiation downstream from caspase-8 activation. J Biol Chem. 1999; 274(20):14255-14261. (Biology). 查看参考
      2. Cock JG, Tepper AD, de Vries E, van Blitterswijk WJ, Borst J. CD95 (Fas/APO-1) induces ceramide formation and apoptosis in the absence of a functional acid sphingomyelinase. J Biol Chem. 1998; 273(13):7560-7565. (Biology). 查看参考
      3. Fearnhead HO, Rodriguez J, Govek EE, et al. Oncogene-dependent apoptosis is mediated by caspase-9. Proc Natl Acad Sci U S A. 1998; 95(23):13664-13669. (Biology). 查看参考
      4. Muzio M, Chinnaiyan AM, Kischkel FC, et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex. Cell. 1996; 85(6):817-827. (Biology).
      5. Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature. 1997; 386(6624):517-521. (Biology).
      查看所有文件 (5) 查看更少内容
      551244 Rev. 4

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.