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Purified Mouse Anti-DNA Ligase III
商品详情
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human DNA Ligase III aa. 2-115
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
103 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611876 Rev. 1
抗体详情
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7/DNA Ligase III

Cells have evolved DNA repair pathways that are dedicated to the maintenance of DNA integrity. In such pathways, damaged DNA is excised and the resulting gap is filled by DNA polymerase. Human DNA ligases, ligase I, III, and IV, utilize ATP as a co-factor in DNA joining reactions required for base excision and single strand break repair pathways. All DNA ligases contain an RFPR sequence and an active site motif (ASM) on each side of their catalytic domain. The RFPR is required for transfer of an AMP group from the enzyme to the 5'-phosphate terminus of a DNA nick. In addition, DNA ligase III has an N-terminal zinc finger domain (ZFD) that is homoloqous with the zinc fingers found in poly(ADP-ribose) polymerase (PARP). This domain is not required for DNA ligase activity, but enables DNA ligase III to interact with single strand gaps and single strand flaps. During base excision repair (BER), ATP-dependent ligation requires PARP, DNA polymerase β, and DNA ligase III interaction with XRCC1 within the BER complex. Thus, DNA ligase III may contain unique protein sequences that allow interaction and repair of specific types of DNA damage.

611876 Rev. 1
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611876 Rev.1
报价单和参考
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研发参考 (4)

  1. Chen J, Tomkinson AE, Ramos W. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination. Mol Cell Biol. 1995; 15(10):5412-5422. (Biology). 查看参考
  2. Mackey ZB, Niedergang C, Murcia JM. DNA ligase III is recruited to DNA strand breaks by a zinc finger motif homologous to that of poly(ADP-ribose) polymerase. Identification of two functionally distinct DNA binding regions within DNA ligase III. J Biol Chem. 1999; 274(31):21679-21687. (Biology). 查看参考
  3. Oei SL, Ziegler M. ATP for the DNA ligation step in base excision repair is generated from poly(ADP-ribose). J Biol Chem. 2000; 275(30):23234-23239. (Biology). 查看参考
  4. Taylor RM, Whitehouse CJ, Caldecott KW. The DNA ligase III zinc finger stimulates binding to DNA secondary structure and promotes end joining. Nucleic Acids Res. 2000; 28(18):3558-3563. (Biology). 查看参考
查看所有文件 (4) 查看更少内容
611876 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.