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Western blot analysis of Desmoglein on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti-Desmoglein antibody.
BD Transduction Laboratories™ Purified Mouse Anti-Desmoglein
监管状态图例
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准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Desmoglein (Dsg1) is a transmembrane glycoprotein found in desmosome junctions. Desmosomes and adherens junctions are plasma membrane specializations involved in cell-cell adhesion. They serve as membrane anchorage domains for intermediate filaments and microfilaments, respectively. Actin microfilaments anchor at adherens junctions, which occur in many sizes, shapes, and positions. These junctions generally contain E- or N-Cadherin whose cytoplasmic region is associated with cytoplasmic proteins such as α- and β- catenin, vinculin, α-actinin, and radixin. Intermediate filaments (IFs) are anchored at desmosomes which contain desmosome-specific cadherin family members, known as desmocollin (Dsc) and desmoglein. These proteins associate with cytoplasmic proteins such as desmoplakin and plakoglobin (γ-Catenin). Plakoglobin is the only junctional protein found at both desmosomes and adherens junctions and, therefore, plays a crucial role in cell adhesion.
研发参考 (5)
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Mathur M, Goodwin L, Cowin P. Interactions of the cytoplasmic domain of the desmosomal cadherin Dsg1 with plakoglobin. J Biol Chem. 1994; 269(19):14075-14080. (Biology). 查看参考
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Peltonen S, Hentula M, Hägg P. A novel component of epidermal cell-matrix and cell-cell contacts: transmembrane protein type XIII collagen. J Invest Dermatol. 1999; 113(4):635-642. (Biology: Immunohistochemistry). 查看参考
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Stewart DB, Barth AI, Nelson WJ. Differential regulation of endogenous cadherin expression in Madin-Darby canine kidney cells by cell-cell adhesion and activation of beta -catenin signaling. J Biol Chem. 2000; 275(27):20707-20716. (Biology: Western blot). 查看参考
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Wheeler GN, Parker AE, Thomas CL. Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules. Proc Natl Acad Sci U S A. 1991; 88(11):4796-4800. (Biology). 查看参考
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Wine RN, Chapin RE. Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat. J Androl. 1999; 20(2):198-213. (Biology: Immunohistochemistry, Western blot). 查看参考
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