BD Transduction Laboratories™ Purified Mouse Anti-γ-Catenin
克隆 15/γ-Catenin (RUO)
Western blot analysis of γ-Catenin on a HeLa lysate. Lane 1: 1:2000, lane 2: 1:4000, lane 3: 1:8000 dilution of the anti- γ-Catenin antibody.
Immunofluorescence staining of MCF7 cells.
Western blot analysis of γ-Catenin on a HeLa lysate. Lane 1: 1:2000, lane 2: 1:4000, lane 3: 1:8000 dilution of the anti- γ-Catenin antibody.
Immunofluorescence staining of MCF7 cells.
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
配套商品
γ-Catenin (plakoglobin) was identified as a compenent of desmosomes where it associates with desmoglein. γ-Catenin and β-Catenin are closely related proteins that have significant homology with the Drosophila armadillo protein. In addition to complexing with E-Cadherin, γ-Catenin and β-Catenin have been observed in association with the intracellular domain of N-Cadherin. It has been proposed that one molecule of α-Catenin and at least one molecule of β-Catenin and γ-Catenin simultaneously bind to a single cadherin molecule. A 19 amino acid sequence of desmoglein (Dsg1) was found to be critical for binding of γ-Catenin. This region has significant homology to the catenin-binding domain of classical cadherins, thus suggesting a common mechanism for γ-Catenin localization at both adherens junctions and desmosomes.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Mary S, Charrasse S, Meriane M, et al. Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism. Mol Biol Cell. 2002; 13(1):285-301. (Biology: Western blot). 查看参考
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McCrea PD, Turck CW, Gumbiner B. A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin. Science. 1991; 254(5036):1359-1361. (Biology). 查看参考
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Merritt AJ, Berika MY, Zhai W, et al. Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation. Mol Cell Biol. 2002; 22(16):5846-5858. (Biology: Immunohistochemistry). 查看参考
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Muller T, Choidas A, Reichmann E, Ullrich A. Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration. J Biol Chem. 1999; 274(15):10173-10183. (Biology: Immunoprecipitation, Western blot). 查看参考
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Peng YF, Mandai K, Nakanishi H, et al. Restoration of E-cadherin-based cell-cell adhesion by overexpression of nectin in HSC-39 cells, a human signet ring cell gastric cancer cell line. Oncogene. 2002; 21(26):4108-4119. (Biology: Immunofluorescence, Western blot). 查看参考
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