BD Transduction Laboratories™ Purified Mouse Anti-AKAP149
克隆 6/AKAP149 (RUO)
Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- AKAP149 antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- AKAP149 antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
配套商品
The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Dell'Acqua ML, Scott JD. Protein kinase A anchoring. J Biol Chem. 1997; 272(20):12881-12884. (Biology). 查看参考
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Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
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Steen RL, Collas P. Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression. J Cell Biol. 2001; 153(3):621-626. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
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Tanji C, Yamamoto H, Yorioka N, Kohno N, Kikuchi K, Kikuchi A. A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta. J Biol Chem. 2002; 277(40):36955-36961. (Biology: Immunoprecipitation, Western blot). 查看参考
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Trendelenburg G, Hummel M, Riecken EO, Hanski C. Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain. Biochem Biophys Res Commun. 1996; 225(1):313-319. (Biology). 查看参考
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