Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Website Feedback
      Alert Icon
      Purified Mouse Anti- AIM-1
      A78720_611083_IMAGE1.png

      Western blot analysis of AIM-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- AIM-1 antibody.

      A78720_611083_image2.png

      Immunofluorescence staining of human endothelial cells.

      A78720_611083_IMAGE1.png A78720_611083_image2.png Blue-Cap-White-Label.jpg

      Western blot analysis of AIM-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- AIM-1 antibody.

      Immunofluorescence staining of human endothelial cells.

      商品详情
      Down Arrow Up Arrow


      BD Transduction Laboratories™
      Aurora B; Aurora and Ipl1-like midbody associated protein
      Human (QC Testing), Mouse, Rat (Tested in Development)
      Mouse IgG1
      Rat AIM-1 aa. 2-124
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
      41 kDa
      250 µg/ml
      AB_398396
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      611083 Rev. 2
      抗体详情
      Down Arrow Up Arrow
      6/AIM-1

      The mitotic phase of the cell cycle is a complex process that ensures the fidelity of chromosome segregation. During the final stage of mitosis (telophase), segregated chromosomes become less condense and nuclear membranes surround the two sets of daughter chromosomes. Simultaneously, the separation and segregation of the cytoplasm (cytokinesis) ensures complete division and formation of two identical daughter cells. Regulation of cytokinesis is poorly understood and errors in this process can lead to cell death or oncogenesis. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae Ipl1 kinase are highly homologous and are required for progression through mitosis. Their mammalian homolog AIM-1 (also known as Aurora and Ipl1-like midbody associated protein) accumulates at the G2/M interface. During late anaphase, AIM-1 is found at the equator of central spindles. However, during telophase and cytokinesis, it is found at the midbody. Although over-expression of a kinase-inactive AIM-1 mutant disrupts formation of the cleavage furrow, nuclear division is unaffected. Thus, it is thought that AIM-1 is essential for cleavage furrowing and the onset of cytokinesis.

      611083 Rev. 2
      格式详情
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      611083 Rev.2
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "611083" on CiteAb

      研发参考 (5)

      1. Chen J, Jin S, Tahir SK. Survivin enhances Aurora-B kinase activity and localizes Aurora-B in human cells. J Biol Chem. 2003; 278(7):486-490. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
      2. Lange BM, Rebollo E, Herold A, Gonzalez C. Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes. EMBO J. 2002; 21(20):5364-5374. (Biology: Immunoprecipitation). 查看参考
      3. Tatsuka M, Katayama H, Ota T. Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res. 1998; 58(21):4811-4816. (Biology). 查看参考
      4. Terada Y, Tatsuka M, Suzuki F, Yasuda Y, Fujita S, Otsu M. AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 1998; 17(3):667-676. (Biology). 查看参考
      5. Trinkle-Mulcahy L, Andrews PD, Wickramasinghe S, et al. Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle. Mol Biol Cell. 2003; 14(1):107-117. (Biology: Immunofluorescence). 查看参考
      查看所有文件 (5) 查看更少内容
      611083 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.