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HRP Protein A
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BD Transduction Laboratories™
Western blot (Routinely Tested)
1.0 mg/ml
1 mg/ml in 50% glycerol, 20mM sodium phosphate pH 7.5, 150 mM NaCl.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
610438 Rev. 1
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Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes. It can also be utilized for antibody purification. As a horseradish peroxidase conjugate, protein A can be used instead of secondary antibody detection methods in Western blotting with polyclonal antibodies and with IgG2a and IgG2b mouse monoclonal antibodies.  

Horseradish peroxidase (HRP) was coupled to protein A at a concentration of 4 mg/mg of protein A using the periodate method. For the detection of antigen using polyclonal antibodies in Western blotting, a concentration of 0.5 µg/ml (1:2000 dilution) is suggested.

610438 Rev. 1
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Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
610438 Rev.1
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610438 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.