HRP Mouse Anti-Phosphotyrosine

BD Transduction Laboratories™ HRP Mouse Anti-Phosphotyrosine

Name: HRP Mouse Anti-Phosphotyrosine
Brand: HRP Mouse Anti-Phosphotyrosine
SKU: 610011

克隆 PY20 (RUO)

Western blot analysis of phosphotyrosine on A431 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-phosphotyrosine, PY20.

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实验方案

科学资源

目录编号:610011

您的价格:登录

目录价格:¥6,161.00

单位:EA (1 Each)

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商品详情

Brand: BD Transduction Laboratories™

Reactivity: Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog (Tested in Development)

Isotype: Mouse IgG2b

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Not Recommended)

Concentration: 250 µg/ml

RRID: AB_397433

Storage Buffer: Aqueous buffered solution containing BSA and glycerol.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed. Store undiluted at -20°C.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

数据表

技术数据表（TDS）

610011 Rev. 1

抗体详情

PY20

Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.

Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

610011 Rev. 1

格式详情

HRP

Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.

格式: HRP

610011 Rev.1

报价单和参考

View Development References

研发参考 (5)

Alderton F, Rakhit S, Kong KC, et al. Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells. J Biol Chem. 2001; 276(30):28578-28585. (Clone-specific: Western blot). 查看参考
Borges E, Jan Y, Ruoslahti E. Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain. J Biol Chem. 2000; 275(51):39867-39873. (Clone-specific: Western blot). 查看参考
Goitsuka R, Tatsuno A, Ishiai M, Kurosaki T, Kitamura D. MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT. J Biol Chem. 2001; 276(38):36043-36050. (Clone-specific: Western blot). 查看参考
Hemmeryckx B, Reichert A, Watanabe M, et al. BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl. Oncogene. 2002; 21(20):3225-3231. (Clone-specific: Western blot). 查看参考
Smith AJ, Surviladze Z, Gaudet EA, Backer JM, Mitchell CA, Wilson BS. p110beta and p110delta phosphatidylinositol 3-kinases up-regulate Fc(epsilon)RI-activated Ca2+ influx by enhancing inositol 1,4,5-trisphosphate production. J Biol Chem. 2001; 276(20):17213-17220. (Clone-specific: Western blot). 查看参考

查看所有文件 (5)

610011 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.