Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
Products

Link Arrow
解决方案

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu

Dear Customer,  If you experience any text readability or display issues on our website, please update your web browser to the latest version

See More

Close
Website Feedback
Alert Icon
P54320_610620_image1.png

Western blot analysis of Paxillin of human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of anti-Paxillin.

P54320_610620_image2.png

Immunofluorescent staining of WI38 cells with anti-Paxillin.

P54320_610620_image1.png P54320_610620_image2.png Blue-Cap-White-Label.jpg

Western blot analysis of Paxillin of human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of anti-Paxillin.

Immunofluorescent staining of WI38 cells with anti-Paxillin.

商品详情
Down Arrow Up Arrow


BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Chicken Paxillin aa. 1-557
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
68 kDa
250 µg/ml
AB_397952
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
显示较多信息 blue down arrow 显示较少信息 blue up arrow
610619 Rev. 1
抗体详情
Down Arrow Up Arrow
165/Paxillin

Paxillin, a focal adhesion protein, is a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. This is consisent with the possibility that paxillin is involved in the regulation of cell morphology. Additionally, because of its SH3 binding domain, paxillin associates tightly with FAK and Crk in an extracellular matrix-independent manner. Paxillin was initially detected in fibroblasts, and its phosphorylation may be important during neurite extension during differentiation.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610619 Rev. 1
格式详情
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610619 Rev.1
报价单和参考
Down Arrow Up Arrow
View product citations for antibody "610619" on CiteAb

研发参考 (5)

  1. Herreros L, Rodriguez-Fernandez JL, Brown MC. Paxillin localizes to the lymphocyte microtubule organizing center and associates with the microtubule cytoskeleton. J Biol Chem. 1995; 270(34):5039-5047. (Clone-specific: Immunofluorescence). 查看参考
  2. Laukaitis CM, Webb DJ, Donais K, Horwitz AF. Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. J Cell Biol. 2001; 153(7):1427-1440. (Clone-specific: Immunofluorescence). 查看参考
  3. Muller G, Jung C, Wied S, Welte S, Jordan H, Frick W. Redistribution of glycolipid raft domain components induces insulin-mimetic signaling in rat adipocytes. Mol Cell Biol. 2001; 21(14):4553-4567. (Clone-specific: Western blot). 查看参考
  4. Salgia R, Li JL, Lo SH, et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem. 1995; 270(10):5039-5047. (Biology). 查看参考
  5. Turner CE, Glenney JR Jr, Burridge K. Paxillin: a new vinculin-binding protein present in focal adhesions. J Cell Biol. 1990; 111(3):1059-1068. (Biology). 查看参考
查看所有文件 (5) 查看更少内容
610619 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.