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      Purified Mouse Anti-Mouse TFE3
      Purified Mouse Anti-Mouse TFE3
      Western blot analysis of TFE3. Lysate from WI-38 cells was probed with anti- TFE3 (clone G138-312) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). TFE3 is identified as a band of 50 kDa.
      Purified Mouse Anti-Mouse TFE3
      Western blot analysis of TFE3. Lysate from WI-38 cells was probed with anti- TFE3 (clone G138-312) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). TFE3 is identified as a band of 50 kDa.
      商品详情
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      BD Pharmingen™
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG1
      TFE3-L Protein
      Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Reported)
      50 kDa
      0.5 mg/ml
      AB_395332
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Applications include western blot analysis (0.25-1.0 µg/ml). WI-38 cells are suggested as a positive control. Other applications include gel shift (supershifts) and immunoprecipitation, which are not routinely tested by BD Biosciences Pharmingen. The antibody has been used to detect in vitro translated TFE3-L and TFE3-L, expressed as a recombinant protein in bacteria. In gel shift assays, using µE3 as a probe, this antibody supershifts the complex.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      554263 Rev. 9
      抗体详情
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      G138-312

      TFE3 is a ubiquitously expressed 50 kDa transcription factor containing both basic helix-loop-helix (bHLH) and leucine zipper (ZIP) motifs. It was initially identified as a protein which bound to the µE3 site in the immunoglobulin heavy chain enhancer.  In addition, it binds to the MLTF/USF site in the adenovirus major late promoter. Upon binding as a dimer, it induces a minor groove-oriented bend in the DNA, similar to other HLH proteins such as TFEB, USF, myc, and max.  Binding results in transcriptional activation and is mediated through the basic region located just N-terminal to the HLH domain. Clone G138-312 recognizes TFE3. The antibody was raised against a bacterially expressed TFE3-L protein consisting of 327 amino acids encoded by nucleotides 361-2469 of the cloned cDNA.  

      554263 Rev. 9
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554263 Rev.9
      报价单和参考
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      View product citations for antibody "554263" on CiteAb

      研发参考 (5)

      1. Beckmann H, Su LK, Kadesch T. TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif. Genes Dev. 1990; 4(2):167-179. (Biology). 查看参考
      2. Fisher DE, Parent LA, Sharp PA. Myc/Max and other helix-loop-helix/leucine zipper proteins bend DNA toward the minor groove. Proc Natl Acad Sci U S A. 1992; 89(24):11779-11783. (Biology). 查看参考
      3. Murre C, McCaw PS, Baltimore D. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell. 1989; 56(5):777-783. (Clone-specific: Activation). 查看参考
      4. Roman C, Matera AG, Cooper C. mTFE3, an X-linked transcriptional activator containing basic helix-loop-helix and zipper domains, utilizes the zipper to stabilize both DNA binding and multimerization. Mol Cell Biol. 1992; 12(2):817-827. (Biology). 查看参考
      5. Zhao GQ, Zhao Q, Zhou X, Mattei MG, de Crombrugghe B. TFEC, a basic helix-loop-helix protein, forms heterodimers with TFE3 and inhibits TFE3-dependent transcription activation. Mol Cell Biol. 1993; 13(8):4505-4512. (Clone-specific). 查看参考
      查看所有文件 (5) 查看更少内容
      554263 Rev. 9

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.