Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
Profile Icon Profile Icon
登录/注册 UserName
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Human MLH1
      Purified Mouse Anti-Human MLH1
      Immunoprecipitation of MLH1. Two different monoclonal antibodies were used to immunoprecipitate MLH1 from equal amounts of whole cell extracts of NIH/3T3 mouse cells. Lane 1, a strong MLH1 band was seen with clone G168-728. Lane 2, only a faint band was seen using clone G168-15. Lane 3, an IgG2a isotype control.
      Purified Mouse Anti-Human MLH1
      Western blot analysis of MLH1. 30 µg of 293 cell lysate per lane was probed with 3 µg/ml (lane 1) or 1 µg/ml (lane 2) of anti- MLH1 antibody (clone G168-728).
      Purified Mouse Anti-Human MLH1 Purified Mouse Anti-Human MLH1
      Immunoprecipitation of MLH1. Two different monoclonal antibodies were used to immunoprecipitate MLH1 from equal amounts of whole cell extracts of NIH/3T3 mouse cells. Lane 1, a strong MLH1 band was seen with clone G168-728. Lane 2, only a faint band was seen using clone G168-15. Lane 3, an IgG2a isotype control.
      Western blot analysis of MLH1. 30 µg of 293 cell lysate per lane was probed with 3 µg/ml (lane 1) or 1 µg/ml (lane 2) of anti- MLH1 antibody (clone G168-728).
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG2a, κ
      Recombinant Human MLH
      Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
      80-85 kDa
      0.5 mg/ml
      AB_395227
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Applications include immunoprecipitation (2 µg/1x106 cells) and western blot analysis (1-3 µg/ml). MCF-7 human breast carcinoma (ATCC HTB-22), 293 adenovirustransformed human kidney (ATCC CRL-1673), and NIH/3T3 mouse fibroblast (ATCC CRL-1658) cells are suggested as positive controls. Clone G168-15 (Cat. No. 13271A) is suggested for immunohistochemical analysis of MLH1; clone G168-15 may also be stronger for western blot analysis than clone G168-728 in some assay systems.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      554073 Rev. 7
      抗体详情
      Down Arrow Up Arrow
      G168-728

      The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. DNA repair enzymes are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL.  MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex, it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair.  The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen.  

      554073 Rev. 7
      格式详情
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554073 Rev.7
      报价单和参考
      Down Arrow Up Arrow

      研发参考 (3)

      1. Baker SM, Plug AW, Prolla TA. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet. 1996; 13(3):336-342. (Clone-specific: Immunoprecipitation). 查看参考
      2. Prolla TA, Christie DM, Liskay RM. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene. Mol Cell Biol. 1994; 14(1):407-415. (Biology). 查看参考
      3. Prolla TA, Pang Q, Alani E, Kolodner RD, Liskay RM. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994; 265(5175):1091-1093. (Biology). 查看参考
      554073 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback