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Purified Mouse Anti-Human FLI-1
Purified Mouse Anti-Human FLI-1
Western blot analysis of Fli-1. Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). Fli-1 is identified as a band of ~50 kDa.
Western blot analysis of Fli-1. Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). Fli-1 is identified as a band of ~50 kDa.
商品详情
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2a
Fli-1 ets Domain Fusion Protein
Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Tested During Development)
50 kDa
0.5 mg/ml
AB_395334
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推荐的实验流程

Applications include western blot analysis (0.5-2 µg/ml). Additional applications not routinely tested at BD Biosciences Pharmingen include gel shift and immunoprecipitation. Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. In gel shift assays this antibody supershifts complexes of Fli-1 and DNA probe.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554267 Rev. 5
抗体详情
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G146-254

Fli-1 is a 50 kDa ets-related transcription factor.  Like other ets-related proteins it binds to consensus sites in DNA through a 85 amino acid ets domain in the carboxyl region of the protein.  Fli-1 is associated with Ewing sarcoma through at (11;22)(q24;q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli-1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself.  The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to trans-activate the promoter for c-myc. C-myc is known to be elevated in Ewing sarcomas.  Clone G146-254 was raised against a bacterially expressed Fli-1 ets domain fusion protein.

554267 Rev. 5
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554267 Rev.5
报价单和参考
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研发参考 (2)

  1. Bailly RA, Bosselut R, Zucman J. DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma. Mol Cell Biol. 1994; 14(5):3230-3241. (Biology). 查看参考
  2. May WA, Lessnick SL, Braun BS. The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1. Mol Cell Biol. 1993; 13(12):7393-7398. (Biology). 查看参考
554267 Rev. 5

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.