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      ATAC-Seq Amplification Kit

      BD Rhapsody™ ATAC-Seq Amplification Kit

      商品详情
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      BD Rhapsody™
      Single cell multiomics experiments on the BD Rhapsody™ platform (Tested During Development)
      RUO


      描述

      The BD Rhapsody™ ATAC-Seq Amplification Kit provides the necessary reagents to support the preparation of single-cell standalone ATAC-Seq libraries from the nuclei prepared using the BD Rhapsody™ ATAC-Seq Tagmentation and Supplemental Reagents Kit (sold separately) and captured on the BD Rhapsody™ Single-Cell Analysis system. For detailed instructions on the tagmentation step and single nuclei capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ HT Xpress system, refer to the protocols listed in the Recommended Assay Procedure section. Optimized for use with Rhapsody Enhanced Cell Capture Beads V3, the BD Rhapsody™ ATAC-Seq Amplification Kit provides all the reagents required for the ligation and gap filling steps performed on the transposed fragments captured from the nuclei loaded onto the BD Rhapsody™ Single-Cell Analysis system. This kit also provides a set of ready-to-use index primers to prepare 8 unique single-indexed libraries from the DNA fragments denatured off the beads after the gap-filling step. For detailed instructions on the ligation, gap-filling, and library preparation steps, refer to the protocol listed in the Recommended Assay Procedure section.

      推荐的实验流程

      After retrieving and washing the Cell Capture Beads from the Rhapsody Cartridge, a ligation mix, prepared using the reagents provided in the kit, is added to the Beads to ligate the double-stranded DNA fragments to the bead oligos. Following that, a gap-filling mix consisting of the Gap-Filling Enzyme and other reagents provided in the kit is prepared and added to the beads to repair the tagmentase-induced nicks in the transposed fragments ligated to the bead oligos.

      For detailed instructions on the tagmentation step, splint-bead generation, ligation, gap-filling, and preparation of standalone ATAC-Seq libraries, refer to the protocol Single-Cell ATAC Library Preparation Protocol (Doc ID 23-24473).

      For instructions on how to use the BD Rhapsody™ Single-Cell Analysis system, refer to the BD Rhapsody™ HT Xpress Single-Cell Analysis System Instrument User Guide (Doc ID 23-24257) or the BD Rhapsody™ HT Xpress System Instrument User Guide for Scanner-free Workflow (Doc ID 23-24256).

      For sample preparation prior to the library construction, refer to the protocol BD Rhapsody™ System Preparing Single Cell Suspensions Protocol (Doc ID 23-24126) and Rhapsody HT Single-Cell Capture and Analysis System Single-Cell Capture and cDNA Synthesis Protocol (Doc ID 23-24252).

      For information on the bioinformatic pipeline for the ATAC-Seq assay, refer to BD® Single-Cell Multiomics Bioinformatics Handbook (Doc ID 54169).

      For safety information, see the BD Rhapsody ™ Single-Cell Analysis System Instrument User Guide (Doc ID 23-24257).

      Use standard laboratory safety protocols. Read and understand the safety data sheets (SDSs) before handling chemicals. To obtain SDSs, go to regdocs.bd.com or contact BD Biosciences technical support at scomix@bdscomix.bd.com.

      Warning: All biological specimens and materials are considered biohazardous so that personal protection should be exercised coming into contact with them. Handle these as if they are capable of transmitting infection and dispose of them with proper precautions in accordance with federal, state, and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.

      Danger: PCR Master Mix contains 5-10 % Tris base, CAS number 77-86-1, EC number 201-064-4 and 1-5 % tetramethylammonium chloride, CAS number 75-57-0, EC number 200-880-8. These reagents are classified as hazardous according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS).

      Danger

      Causes skin irritation.

      Cause damage to organs (Central Nervous System) via ingestion.

      Harmful if swallowed.

      Prevention

      Wash skin thoroughly after handling

      Wear protective gloves/protective clothing/eye protection/face protection/hearing protection

      Do not breathe dust/fume/gas/mist/vapors/spray

      Do not eat, drink or smoke when using this product

      Response

      IF ON SKIN: Wash with plenty of soap and water.

      Take off contaminated clothing and wash it before reuse.

      If skin irritation occurs: Get medical advice/attention.

      Specific treatment (see Safety Data Sheet)

      IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell

      Rinse mouth

      IF exposed or concerned: Call a POISON CENTER or physician

      Disposal

      Store locked up.

      Dispose of contents/container to an approved facility in accordance with local, regional, national and international regulations.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      4. For U.S. patents that may apply, see bd.com/patents.
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      571356 Rev. 1
      组件
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      描述 数量/尺寸 零件号 EntrezGene ID
      Ligation Buffer N/A 51-9023094 N/A
      Ligase N/A 51-9023093 N/A
      Nuclease Free Water N/A 51-9023086 N/A
      Gap-Filling Enzyme N/A 51-9023095 N/A
      Gap-Filling Buffer N/A 51-9023096 N/A
      ATAC-Seq Library Forward Primer N/A 51-9023097 N/A
      ATAC-Seq Library Reverse Primer 1 N/A 51-9023098 N/A
      ATAC-Seq Library Reverse Primer 2 N/A 51-9023099 N/A
      ATAC-Seq Library Reverse Primer 3 N/A 51-9023100 N/A
      ATAC-Seq Library Reverse Primer 4 N/A 51-9023101 N/A
      ATAC-Seq Library Reverse Primer 5 N/A 51-9023102 N/A
      ATAC-Seq Library Reverse Primer 6 N/A 51-9023103 N/A
      ATAC-Seq Library Reverse Primer 7 N/A 51-9023108 N/A
      ATAC-Seq Library Reverse Primer 8 N/A 51-9023109 N/A
      dNTP N/A 51-9023110 N/A
      Gap-Filling Enhancer N/A 51-9023111 N/A
      Elution Buffer N/A 51-9023107 N/A
      Bead Resuspension Buffer N/A 51-9023126 N/A
      PCR MasterMix N/A 51-9024048 N/A
      571356 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.