Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Website Feedback
      Alert Icon
      Oligo Rat Anti-Mouse Delta-Like Protein 4

      BD™ AbSeq Oligo Rat Anti-Mouse Delta-Like Protein 4

      克隆 9A1.5 (RUO)

      商品详情
      Down Arrow Up Arrow


      BD™ AbSeq
      Delta like-4; Delta4; Dll4; DL4; DL-4
      54485
      2 µl
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Mouse (Tested in Development)
      Single Cell 3' Sequencing (Qualified)
      GGATAGAGGTGTAGTGAATAATGGATAGTCGGCAGT
      AMM2262
      Mouse DL4 Extracellular Domain Recombinant Protein
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO
      Rat


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      推荐的实验流程

      Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.

      BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      4. Illumina is a trademark of Illumina, Inc.
      5. Please refer to bd.com/genomics-resources for technical protocols.
      6. This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For U.S. patents that may apply, see bd.com/patents.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      940493 Rev. 2
      抗体详情
      Down Arrow Up Arrow
      9A1.5

      The 9A1.5 monoclonal antibody specifically binds to an extracellular domain of Delta-Like Protein 4 that is encoded by the Dll4 gene. Delta-Like Protein 4 is a type 1 transmembrane glycoprotein that is also known as Delta-like 4, Delta-like Ligand 4, Delta4 and DL4. It is a member of the Delta/Serrate/Jagged Family of Notch ligands. Notch ligands are classified by the presence of specific structural motifs including an N-terminal Delta-Serrate-LAG-2 (DSL) domain necessary for Notch binding, EGF repeats, and the DOS domain (a specialized EGF repeat). The Notch family of transmembrane receptors and their ligands control cell-fate "decisions" during the development of many organs in a wide variety of animal species. Delta-Like Protein 4 activates cellular signaling pathways by binding to Notch1 and Notch4 receptors. It is involved in embryonic vascular development and tumor angiogenesis, and is induced by vascular endothelial growth factor (VEGF)-A and hypoxia. Delta-Like Protein 4 and Notch receptor signaling are also intimately involved in the regulation of innate and adaptive immunity. Delta-Like Protein 4 is highly expressed by cortical thymic epithelial cells (TEC). Interaction between Delta-Like Protein 4-positive TEC and Notch1 expressed by T cell precursors drives T cell lineage commitment, expansion and maturation within the thymus. Delta-Like Protein 4 is also expressed by dendritic cells and plays a role in peripheral T helper cell differentiation. Blocking of Delta-Like Protein 4 and Notch receptor interactions serves to diminish autoimmune diseases in mouse model systems.

      940493 Rev. 2
      格式详情
      Down Arrow Up Arrow
      Ab-Oligo
      The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD® AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD® AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.NOTE: The BD Rhapsody™ Single-Cell Analysis System must be used with the BD Rhapsody™ Express Instrument.
      Ab-Oligo
      940493 Rev.2
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "940493" on CiteAb

      研发参考 (7)

      1. Fiorini E, Ferrero I, Merck E, et al. Cutting edge: thymic crosstalk regulates delta-like 4 expression on cortical epithelial cells. J Immunol. 2008; 181(12):8199-8203. (Immunogen: ELISA, Fluorescence microscopy, Immunofluorescence). 查看参考
      2. Kassner N, Krueger M, Yagita H, et al. Cutting edge: Plasmacytoid dendritic cells induce IL-10 production in T cells via the Delta-like-4/Notch axis. J Immunol. 2010; 184(2):550-554. (Biology). 查看参考
      3. Koch U, Fiorini E, Benedito R, et al. Delta-like 4 is the essential, nonredundant ligand for Notch1 during thymic T cell lineage commitment. J Exp Med. 2008; 205(11):2515-2523. (Clone-specific: Flow cytometry). 查看参考
      4. Mori M, Yoneyama M, Ito T, Takahashi K, Inagaki F, Fujita T. Identification of Ser-386 of interferon regulatory factor 3 as critical target for inducible phosphorylation that determines activation. J Biol Chem. 2004; 279(11):9698-9702. (Biology). 查看参考
      5. Radtke F, MacDonald HR, Tacchini-Cottier F. Regulation of innate and adaptive immunity by Notch. Nat Rev Immunol. 2013; 13(6):427-437. (Biology). 查看参考
      6. Shutter JR, Scully S, Fan W, et al. Dll4, a novel Notch ligand expressed in arterial endothelium. Genes Dev. 2000; 14:1313-1318. (Biology). 查看参考
      7. Shutter JR, Scully S, Fan W, et al. Molecular cloning of delta-4, a new mouse and human Notch ligand. J Biochem (Tokyo). 2001; 129(1):27-34. (Biology). 查看参考
      查看所有文件 (7) 查看更少内容
      940493 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.