The 1D3/CD84 monoclonal antibody specifically recognizes CD84 which is also known as Signaling lymphocytic activation molecule 5 (SLAM family member 5 or Slamf5). CD84 is a single-pass type I transmembrane glycoprotein that is encoded by Cd84 (CD84 antigen) which belongs to the SLAM family within the CD2-subset of the immunoglobulin superfamily (IgSF) of cell surface receptors. CD84 is comprised of an extracellular region with one N-terminal Ig-like V-type (IgV) domain followed by one Ig-like C2-type (IgC2) domain. Its cytoplasmic tail contains two immunoreceptor tyrosine-based switch motifs (ITSMs) and serves as a docking site for activating adaptor molecules, SLAM-associated protein (SAP) or EWS-activated transcript 2 (EAT-2), or inhibitory SH2-binding phosphatases. CD84 is variably expressed on B cells, T cells, NK cells, monocytes, macrophages, dendritic cells, granulocytes, mast cells, hematopoietic progenitor cells, and platelets. CD84 is a homotypic adhesion molecule that can function as a coreceptor in the regulation of innate and adaptive immune responses including the enhancement of T cell proliferation and cytokine production.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.