准备和存储
推荐的实验流程
Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.
BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.
商品通知
- This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Illumina is a trademark of Illumina, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to bd.com/genomics-resources for technical protocols.
- For U.S. patents that may apply, see bd.com/patents.
配套商品
The 4G2 monoclonal antibody specifically recognizes CD319 which is also known as CD2-like receptor activating cytotoxic cells (CRACC), Signaling lymphocytic activation molecule 7 (Slamf7), or novel Ly9. CD319 (CRACC) is a single-pass type I transmembrane glycoprotein that is encoded by Slamf7 (SLAM family member 7) which belongs to the SLAM family within the CD2-subset of the immunoglobulin superfamily (IgSF) of cell surface receptors. This receptor is comprised of an extracellular region with one N-terminal Ig-like V-type (IgV) domain followed by one Ig-like C2-type (IgC2) domain. Its cytoplasmic tail contains two immunoreceptor tyrosine-based switch motifs (ITSMs). This receptor is variably expressed on activated CD4+ T cells and CD8+ T cells, NKT cells, NK cells, B cells, monocytes, macrophages, and dendritic cells. CD319 (CRACC) serves as a homotypic adhesion molecule that can self-associate and trigger cellular activation, such as cell-mediated cytotoxicity, when its cytoplasmic domains are associated with adaptor molecules including EWS-activated transcript 2 (EAT-2) and possibly EAT-2-related transducer (ERT). In cells that lack EAT-2, CD319 (CRACC) may exhibit an inhibitory function.
研发参考 (2)
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Cruz-Munoz ME, Dong Z, Shi X, Zhang S, Veillette A. Influence of CRACC, a SLAM family receptor coupled to the adaptor EAT-2, on natural killer cell function.. Nat Immunol. 2009; 10(3):297-305. (Immunogen). 查看参考
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Guo H, Cruz-Munoz ME, Wu N, Robbins M, Veillette A. Immune cell inhibition by SLAMF7 is mediated by a mechanism requiring src kinases, CD45, and SHIP-1 that is defective in multiple myeloma cells.. Mol Cell Biol. 2015; 35(1):41-51. (Clone-specific). 查看参考
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