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      Purified Mouse Anti-Vimentin
      Purified Mouse Anti-Vimentin
      Western blot analysis of vimentin. HeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified as a band of ~57 kDa.
      Purified Mouse Anti-Vimentin
      Western blot analysis of vimentin. HeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified as a band of ~57 kDa.
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      BD Pharmingen™
      Human (QC Testing), Rat,Mouse,Hamster,Monkey,Dog,Chicken,Rabbit,Cow,Goat (Reported)
      Mouse IgG1
      Purified Cow Lens Vimentin
      Western blot (Routinely Tested), Electron microscopy, Immunofluorescence, Immunohistochemistry (Reported)
      57 kDa
      0.5 mg/ml
      AB_393716
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      550513 Rev. 1
      抗体详情
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      RV202

      Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well to organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Vimentin belongs to the type III category of IF proteins which are expressed in leukocytes, blood vessel endothelial cells, some epithelial cells, and mesenchymal cells. Vimentin is also expressed together with several other IF proteins during the early stages of development. Vimentin is exchanged for the tissue-specific intermediate filament type as differentiation proceeds. Vimentin migrates in SDS/PAGE as a ~57 kDa protein.

      550513 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550513 Rev.1
      报价单和参考
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      View product citations for antibody "550513" on CiteAb

      研发参考 (5)

      1. Broers JL, Carney DN, Klein Rot M, et al . Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci. 1986; 83:37-60. (Clone-specific: Immunofluorescence, Western blot). 查看参考
      2. Lodish HF. Molecular cell biology, 4th ed.. New York: W.H. Freeman; 2000:795-847.
      3. Pieper FR, Schaart G, Krimpenfort PJ, et al. Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol. 1989; 108(3):1009-1024. (Clone-specific: Electron microscopy, Immunofluorescence, Immunohistochemistry, Western blot). 查看参考
      4. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990; 111(5):1971-1985. (Clone-specific: Immunohistochemistry). 查看参考
      5. Viebahn C, Lane EB, Ramaekers FC. Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res. 1988; 253(3):553-562. (Clone-specific: Immunofluorescence). 查看参考
      查看所有文件 (5) 查看更少内容
      550513 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.