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      Purified Rat Anti-Mouse VEGFR-3
      Purified Rat Anti-Mouse VEGFR-3
      Immunohistochemical staining of mouse liver cells. Formalin-fixed, paraffin-embedded sections of Balb/c mouse liver were stained with VEGFR-3 antibody, clone AFL4 (Cat. No. 552857). The antibody labeled endothelial cells of sinusoid. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Magnification 40x.
      Purified Rat Anti-Mouse VEGFR-3
      Immunohistochemical staining of mouse liver cells. Formalin-fixed, paraffin-embedded sections of Balb/c mouse liver were stained with VEGFR-3 antibody, clone AFL4 (Cat. No. 552857). The antibody labeled endothelial cells of sinusoid. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Magnification 40x.
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG2a, κ
      VEGFR-3 extracellular domain
      Immunohistochemistry-paraffin (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
      AB_394498
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Immunohistochemistry: The AFL4 antibody, specific for mouse VEGFR-3, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) is recommended for formalin-fixed, paraffin sections. Tissues tested were mouse spleen, liver, kidney, lung and small intestine. The antibody stains endothelial cells of lymphatic vessels. The isotype control recommended for use with this antibody is purified rat IgG2aκ (Cat. No. 559073). For optimal indirect immunohistochemical staining, the AFL4 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Detection System (Cat. No. 550880). More conveniently, the Anti-Rat Ig HRP Detection Kit (Cat. No. 551013), which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate, can be used for staining.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      552857 Rev. 3
      抗体详情
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      AFL4

      The AFL4 antibody reacts with mouse VEGFR-3, an endothelial-specific receptor tyrosine kinase (RTK) related to VEGF receptors. The immunogen used was the extracellular domain of VEGFR-3, corresponding to nucleotides 45-2354 in the GeneBank L07296. VEGFR-3 is induced in all endothelial cells (EC's) during early embryogenesis, and its expression eventually disappears from the vascular endothelial cells of adult tissues. VEGFR-3 is constitutively expressed in the adult lymphatic endothelium. Although VEGFR-3 is not expressed in adult blood vessels, it is induced in vascular endothelial cells of tumor-bearing tissues. Studies have shown that both VEGF-C and VEGF-D, ligands for VEGFR-3, promote lymphatic vessel proliferation throughout central tumor areas and at their peripheries, demonstrating that such molecules aid in the spread of tumor cells from the primary tumor to local lymph nodes.

      552857 Rev. 3
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      552857 Rev.3
      报价单和参考
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      研发参考 (2)

      1. Kubo H, Fujiwara T, Jussila L, et al. Involvement of vascular endothelial growth factor receptor-3 in maintenance of integrity of endothelial cell lining during tumor angiogenesis. Blood. 2000; 96(2):546-553. (Clone-specific: Western blot). 查看参考
      2. Stacker SA, Caesar C, Baldwin ME, et al. VEGF-D promotes the metastatic spread of tumor cells via the lymphatics. Nat Med. 2001; 7(2):186-191. (Biology). 查看参考
      552857 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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