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      Purified Mouse Anti-Rsk
      R23820_610226_image1.png

      Western blot analysis of Rsk on A431 cells lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Rsk antibody.

      R23820_610226_image2.png

      Immunofluorescent staining of Hs 766T cells.

      R23820_610226_image1.png R23820_610226_image2.png Blue-Cap-White-Label.jpg

      Western blot analysis of Rsk on A431 cells lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Rsk antibody.

      Immunofluorescent staining of Hs 766T cells.

      商品详情
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      BD Transduction Laboratories™
      Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
      Mouse IgG2a
      Human p90[rsk] aa. 1-184
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
      90 kDa
      250 µg/ml
      AB_397622
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610226 Rev. 1
      抗体详情
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      78/RSK

      The p90[rsk] (Rsk) and p70[s6k] kinases were first identified based on their ability to phosphorylate the 40S ribosomal protein S6 in vitro. Both of these enzymes are differentially regulated by serine/threonine phosphorylation in response to mitogenic stimulation. ERK1 and ERK2 have been shown to regulate Rsk activity. Once activated by this phosphorylation, a significant amount of Rsk can be found in the nucleus, suggesting that it has a role in nuclear signaling events. The regulation of nuclear Rsk and ERK activities by growth factors is coordinated with the induction of several early response genes. Rsk has also been shown to be activated by ionizing radiation, presumably through an activated MAP kinase. Studies in Xenopus oocytes and mouse NIH/3T3 cells indicate that inactive Rsk and ERK2 exist in a complex of approximately 110kDa. Upon phosphorylation of Rsk and ERK2, the heterodimer dissociates and at least a portion of these activated kinases translocate to the nucleus.

      610226 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610226 Rev.1
      报价单和参考
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      View product citations for antibody "610226" on CiteAb

      研发参考 (5)

      1. Chung J, Pelech SL, Blenis J. Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity. Proc Natl Acad Sci U S A. 1991; 88(11):4981-4985. (Biology). 查看参考
      2. Hsiao KM, Chou SY, Shih SJ, Ferrell JE Jr. Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo. Proc Natl Acad Sci U S A. 1994; 9(12):5480-5484. (Biology). 查看参考
      3. Majka M, Janowska-Wieczorek A, Ratajczak J. Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis. Blood. 2000; 96(13):4142-4151. (Clone-specific: Western blot). 查看参考
      4. Moor AN, Gan XT, Karmazyn M, Fliegel L. Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium. J Biol Chem. 2001; 276(19):16113-16122. (Clone-specific: Western blot). 查看参考
      5. Morrione A, Navarro M, Romano G. The role of the insulin receptor substrate-1 in the differentiation of rat hippocampal neuronal cells. Oncogene. 2001; 20(35):4842-4852. (Clone-specific: Western blot). 查看参考
      查看所有文件 (5) 查看更少内容
      610226 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.