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      Purified Mouse Anti- Munc-18
      M32320_610337_image1.png

      Western blot analysis of Munc-18 on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti- Munc-18 antibody.

      M32320_610337_image2.png

      Immunohistochemical staining for Munc-18 on a formalin-fixed, paraffin-embedded rat brain section with citrate buffer pretreatment (40X magnification).

      M32320_610337_image1.png M32320_610337_image2.png Blue-Cap-White-Label.jpg

      Western blot analysis of Munc-18 on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti- Munc-18 antibody.

      Immunohistochemical staining for Munc-18 on a formalin-fixed, paraffin-embedded rat brain section with citrate buffer pretreatment (40X magnification).

      商品详情
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      BD Transduction Laboratories™
      Rat (QC Testing), Human, Mouse (Tested in Development)
      Mouse IgG1
      Rat Munc-18-1 aa. 381-567
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
      68 kDa
      250 µg/ml
      AB_397727
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610337 Rev. 1
      抗体详情
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      31/Munc-18

      There are two Munc-18 isoforms: Munc-18-1 and Munc-18-2. Munc-18-1 (n-sec1 or rb-sec1; 68 kDa) is a neuronal protein that binds tightly to syntaxin 1 and functions in synaptic vesicle exocytosis. Munc-18-2 exhibits 63% amino acid sequence identity with Munc-18-1. Although Munc-18-2 is expressed in most tissues, Munc-18-1 is primarily expressed in brain. Both forms of Munc-18 bind tightly to syntaxins 1A, 2, and 3, but not to syntaxin 4. Syntaxin 1A is a plasma membrane protein implicated in synaptic vesicle docking. Following calcium entry into the presynaptic nerve terminal, the neurotransmitter-containing synaptic vesicle fuses with the plasma membrane at a region known as the active zone. Thus, by virtue of its interaction with syntaxin 1A, Munc-18-1 is thought to have an essential function in neurotransmitter release.

      610337 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610337 Rev.1
      报价单和参考
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      View product citations for antibody "610337" on CiteAb

      研发参考 (5)

      1. Fisher RJ, Pevsner J, Burgoyne RD. Control of fusion pore dynamics during exocytosis by Munc18. Science. 2001; 291(5505):875-878. (Biology: Immunofluorescence). 查看参考
      2. Hata Y, Slaughter CA, Sudhof TC. Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin. Nature. 1993; 366(6453):347-351. (Biology). 查看参考
      3. Shuang R, Zhang L, Fletcher A, Groblewski GE, Pevsner J, Stuenkel EL. Regulation of Munc-18/syntaxin 1A interaction by cyclin-dependent kinase 5 in nerve endings. J Biol Chem. 1998; 273(9):4957-4966. (Biology: Immunoprecipitation, Western blot). 查看参考
      4. Torii S, Zhao S, Yi Z, Takeuchi T, Izumi T. Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a. Mol Cell Biol. 2002; 22(15):5518-5526. (Biology: Western blot). 查看参考
      5. Zhang W, Efanov A, Yang SN, et al. Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell. J Biol Chem. 2000; 275(52):41521-41527. (Biology: Western blot). 查看参考
      查看所有文件 (5) 查看更少内容
      610337 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.