BD Transduction Laboratories™ Purified Mouse Anti-Human Villin
克隆 12/Villin (RUO)
Western blot analysis of Villin on HCT-8 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Villin antibody.
Immunofluorescent staining of HCT-8 cells
Western blot analysis of Villin on HCT-8 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Villin antibody.
Immunofluorescent staining of HCT-8 cells
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The isolated intestinal microvillus cytoskeleton (core) consists of four major proteins: actin, villin, fimbrin, and brush border myosin-I. These proteins can assemble in vitro into structures resembling native microvillus cores. Of these components, villin, and brush border myosin-I show tissue-specific expression, so they may be involved in the morphogenesis of intestinal microvilli. Found in association with the microvillar actin bundles of the intestinal brush border, villin is a 95 kDa protein composed of two very similar domains of approximately 44 kDa each, the core, and a C-terminal domain of 8 kDa, the headpiece. The core has been shown to contain villin's Ca[2+] regulated capping, nucleating, and severing activities, but it cannot induce the formation of microfilament bundles without the headpiece. Villin is a useful differentiation marker of early embryogenesis and may be useful in diagnosis and follow-up of colorectal cancers. It has been demonstrated that villin is necessary for both the cytoskeletal and membrane protein organization of a functional brush border.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Friederich E, Pringault E, Arpin M, Louvard D. From the structure to the function of villin, an actin-binding protein of the brush border. Bioessays. 1990; 12(9):403-408. (Biology). 查看参考
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Friederich E, Vancompernolle K, Huet C, et al. An actin-binding site containing a conserved motif of charged amino acid residues is essential for the morphogenic effect of villin. Cell. 1992; 70(1):81-92. (Biology). 查看参考
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McSwine RL, Musch MW, Bookstein C, Xie Y, Rao M, Chang EB. Regulation of apical membrane Na+/H+ exchangers NHE2 and NHE3 in intestinal epithelial cell line C2/bbe. Am J Physiol. 1998; 275(3):C693-C701. (Clone-specific: Western blot). 查看参考
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Nies AT, Konig J, Cui Y, Brom M, Spring H, Keppler D. Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2). Eur J Biochem. 2002; 269(7):1866-1876. (Clone-specific: Immunofluorescence). 查看参考
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Zhai L, Zhao P, Panebra A, Guerrerio AL, Khurana S. Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton. J Biol Chem. 2001; 276(39):36163-36167. (Clone-specific: Western blot). 查看参考
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