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Purified Mouse Anti-Human MSH-2
Purified Mouse Anti-Human MSH-2
Western blot analysis of MSH-2. Lysate from A-431 human epidermal carcinoma cells were probed with anti-MSH2 (clone G219-1129) with concentrations between 1 µg/ml to 0.04 µg/ml (lanes 1-3). MSH-2 is identified at ~102 kD.
Western blot analysis of MSH-2. Lysate from A-431 human epidermal carcinoma cells were probed with anti-MSH2 (clone G219-1129) with concentrations between 1 µg/ml to 0.04 µg/ml (lanes 1-3). MSH-2 is identified at ~102 kD.
商品详情
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1
Recombinant Human MSH2 Protein
Western blot (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin (Tested During Development)
102 kDa
0.5 mg/ml
AB_396378
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推荐的实验流程

For IHC, intestine is suggested as a positive control.  Staining is typically seen in the crypts of Lieberkuhn, similar to that described by others.  Staining is primarily nuclear, but may also be observed in the cytoplasm.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556349 Rev. 8
抗体详情
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G219-1129

The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA.  Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The human homologs of  DNA mismatch repair enzymes MLH1, PMS2, and MSH2 have recently been cloned. G219-1129 recognizes human MSH-2.  A recombinant full-length human MSH2 protein was used as immunogen.

556349 Rev. 8
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556349 Rev.8
报价单和参考
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研发参考 (8)

  1. Cleaver JE. It was a very good year for DNA repair. Cell. 1994; 76(1):1-4. (Biology). 查看参考
  2. Fishel R, Lescoe MK, Rao MR, et al. The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer. Cell. 1993; 75(5):1027-1038. (Biology). 查看参考
  3. Kramer W, Kramer B, Williamson MS, Fogel S. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J Bacteriol. 1989; 171(10):5339-5346. (Biology). 查看参考
  4. Leach FS, Nicolaides NC, Papadopoulos N. Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer. Cell. 1993; 75(6):1215-1225. (Biology). 查看参考
  5. Prolla TA, Christie DM, Liskay RM. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene. Mol Cell Biol. 1994; 14(1):407-415. (Biology). 查看参考
  6. Prolla TA, Pang Q, Alani E, Kolodner RD, Liskay RM. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994; 265(5175):1091-1093. (Biology). 查看参考
  7. Su SS, Modrich P. Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. Proc Natl Acad Sci U S A. 1986; 83(14):5057-5061. (Biology). 查看参考
  8. Wilson TM, Ewel A, Duguid JR. Differential cellular expression of the human MSH2 repair enzyme in small and large intestine. Cancer Res. 1995; 55(22):5146-5150. (Clone-specific: Immunohistochemistry). 查看参考
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556349 Rev. 8

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