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Western blot analysis of Cyclin D1. MCF7 cell lysates (Human breast adenocarcinoma; ATCC HTB-22) was probed with the mouse anti-human Cyclin D1 antibody (clone DCS-6) at 1-2 µg/mL (Lane 1). Cyclin D1 is identified as a band of ~36 kDa.
BD Pharmingen™ Purified Mouse Anti-Human Cyclin D1
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Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.
研发参考 (4)
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Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry. 1996; 25(1):1-13. (Biology).
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Lukas J, Pagano M, Staskova Z, Draetta G, Bartek J. Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines. Oncogene. 1994; 9(3):707-718. (Immunogen: Immunohistochemistry, Immunoprecipitation, Inhibition, Western blot). 查看参考
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Meyerson M, Harlow E. Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 1994; 14(3):2077-2086. (Biology). 查看参考
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de Boer CJ, Schuuring E, Dreef E, et al. Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma. Blood. 1995; 86(7):2715-2723. (Biology: Immunohistochemistry). 查看参考
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