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      Purified Mouse Anti-Human CD8
      74061E_550281_image1.png

      Immunohistochemical staining of CD8+ T lymphocytes. Frozen sections of normal human tonsil was reacted with the HIT8a antibody. CD8+ T lymphocytes can be identified by the intense brown labeling of their cell membranes. Amplification 20X.

      74061E_550281_image1.png Blue-Cap-White-Label.jpg

      Immunohistochemical staining of CD8+ T lymphocytes. Frozen sections of normal human tonsil was reacted with the HIT8a antibody. CD8+ T lymphocytes can be identified by the intense brown labeling of their cell membranes. Amplification 20X.

      商品详情
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
      31.25 µg/ml
      V 5T-CD08.10
      AB_393643
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Immunohistochemistry: The clone HIT8a, specific for human CD8+ T lymphocytes is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains CD8+ T lymphocytes, thymocytes and a small subset of NK cells. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the HIT8a antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available at http://www.bdbiosciences.com/support/resources  The clone HIT8a is not recommended for formalin-fixed paraffin embedded sections.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550372 Rev. 3
      抗体详情
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      HIT8a

      The HIT8a monoclonal antibody specifically binds to CD8a (CD8α). CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of  αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers.  CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. Clones  HIT8a and RPA-T8 are not cross-blocking.

      550372 Rev. 3
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550372 Rev.3
      报价单和参考
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      View product citations for antibody "550372" on CiteAb

      研发参考 (1)

      1. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      550372 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.