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      Purified Mouse Anti-Dynamin II
      D27120_610263_IMAGE1.png

      Western blot analysis of Dynamin II on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2) using 1 µg/mL of the Mouse Anti-Dynamin II antibody.

      D27120_610263_image2.png

      Immunohistochemical staining for Dynamin II on a rat brain section, zinc-fixed paraffin-embedded (40X magnification).

      D27120_610263_IMAGE1.png D27120_610263_image2.png Blue-Cap-White-Label.jpg

      Western blot analysis of Dynamin II on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2) using 1 µg/mL of the Mouse Anti-Dynamin II antibody.

      Immunohistochemical staining for Dynamin II on a rat brain section, zinc-fixed paraffin-embedded (40X magnification).

      商品详情
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      BD Transduction Laboratories™
      Human (QC Testing), Mouse, Rat (Tested in Development)
      Mouse IgG2a
      Rat Dynamin II aa. 274-555
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
      100 kDa
      250 µg/ml
      AB_397659
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      610263 Rev. 2
      抗体详情
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      27/Dynamin II

      Dynamin is a membrane-associated GTPase that binds to GTP, microtubules, and phospholipids. Northern blot analysis has shown dynamin to be present in most tissues but at an amount about 20-fold lower than that in brain. There are at least two distinct dynamin genes in mammals. Transcripts of both dynamin genes are alternatively spliced at two or more sites. The first site is identical in both  dynamins, whereas, the second site differs. The two gene products are known as Dynamin I and Dynamin II and show 79% identity. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. The two proteins are highly homologous in the N-terminal region, while the C-terminal domain shows significant divergence. The GTPase activity of Dynamin I is stimulated several fold by binding to microtubules, phospholipids, and membranous vesicles. Dynamin I is a good substrate of PKC in vitro as well as in vivo in resting nerve terminals. In vitro, phosphorylation occurs at the C-terminus of Dynamin I and this enhances the GTPase activity more than 10-fold. However, Dynamin II is not a substrate of PKC and its activity does not appear to be affected by phosphorylation. These data suggest that the function of the common N-terminal domain between Dynamin I and II may be differentially specified by distinct C-terminal domains.

      610263 Rev. 2
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610263 Rev.2
      报价单和参考
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      View product citations for antibody "610263" on CiteAb

      研发参考 (5)

      1. Cook TA, Urrutia R, McNiven MA. Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues. Proc Natl Acad Sci U S A. 1994; 91(2):644-648. (Biology). 查看参考
      2. Kessels MM, Engqvist-Goldstein AE, Drubin DG. Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation. Mol Biol Cell. 2000; 11(1):393-412. (Biology: Immunofluorescence). 查看参考
      3. Kranenburg O, Verlaan I, Moolenaar WH. Dynamin is required for the activation of mitogen-activated protein (MAP) kinase by MAP kinase kinase. J Biol Chem. 1999; 274(50):35301-35304. (Biology: Western blot). 查看参考
      4. McNiven MA, Kim L, Krueger EW, Orth JD, Cao H, Wong TW. Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape. J Cell Biol. 2000; 151(1):187-198. (Biology: Immunoprecipitation, Western blot). 查看参考
      5. Sontag JM, Fykse EM, Ushkaryov Y, Liu JP, Robinson PJ, Sudhof TC. Differential expression and regulation of multiple dynamins. J Biol Chem. 1994; 269(6):4547-4554. (Biology). 查看参考
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      610263 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.