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Western blot analysis of Vti1b on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Vti1b antibody.
Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).
BD Transduction Laboratories™ Purified Mouse Anti-Vti1b
BD Transduction Laboratories™ Purified Mouse Anti-Vti1b
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Eukaryotic protein trafficking involves packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their contents into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs; VAMP1, VAMP2) and target proteins (t-SNAREs; syntaxin 1, SNAP-25) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. With the identification of all SNARE family members in yeast, the research focus has turned to mammalian cells. Here, sequence analysis has identified additional SNARE proteins, including VTI1a and VTI1b. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. VTI1b is a membrane bound protein whose localization overlaps with the cis/medial Golgi marker mannosidase II and the trans-Golgi marker syntaxin 6. VTI1b interacts with, and disrupts the localization of, syntaxin 5. Thus, VTI1b is thought to function in the regulation of post-Golgi vesicle trafficking.
研发参考 (5)
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Advani RJ, Bae HR, Bock JB, et al. Seven novel mammalian SNARE proteins localize to distinct membrane compartments. J Biol Chem. 1998; 273(17):10317-10324. (Biology). 查看参考
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Chen K, Manga P, Orlow SJ. Pink-eyed dilution protein controls the processing of tyrosinase. Mol Cell Biol. 2002; 13(6):1953-1964. (Biology: Immunofluorescence). 查看参考
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Mallard F, Tang BL, Galli T. Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. J Cell Biol. 2002; 156(4):653-664. (Biology: Western blot). 查看参考
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Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G. Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. J Cell Biol. 2002; 157(1):45-62. (Biology: Western blot). 查看参考
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Yang B, Gonzalez L, Prekeris R, Steegmaier M, Advani RJ, Scheller RH. SNARE interactions are not selective. Implications for membrane fusion specificity. J Biol Chem. 1999; 274(9):5649-5653. (Biology). 查看参考
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