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      Purified Mouse Anti-VASP
      Purified Mouse Anti-VASP
      Western blot analysis of VASP on human endothelial cell lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-VASP.
      Purified Mouse Anti-VASP
      Immunoflurorescent staining on Human Endothelial cells.
      Purified Mouse Anti-VASP Purified Mouse Anti-VASP
      Western blot analysis of VASP on human endothelial cell lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-VASP.
      Immunoflurorescent staining on Human Endothelial cells.
      商品详情
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      BD Transduction Laboratories™
      Human (QC Testing), Dog (Tested in Development)
      Mouse IgG1
      Human VASP aa. 248 - 379
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
      46 kDa
      250 µg/ml
      AB_397822
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610448 Rev. 1
      抗体详情
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      43/VASP

      Vasodilator-stimulated phosphoprotein (VASP), a substrate for cAMP- and cGMP-dependent kinases, is associated with actin filaments, focal adhesions, and dynamic membrane regions. VASP is composed of several distinct domains: a central L-proline-rich domain contains a GPPPPP motif as a single copy and as a three-fold tandem repeat, as well as three conserved phosphorylation sites for cyclic nucleotide-dependent protein kinases (Ser157, Ser239, and Thr278). A C-terminal domain contains a repetitive mixed-charge cluster which is predicted to form an α-helix. The C-terminal domain appears to be responsible for anchoring at focal adhesion sites. VASP has been shown to be a ligand for profilins. Profilins bind to the poly-L-proline motifs of VASP and it is postulated that these two molecules act in concert to convey signal transduction to actin filament formation.

      610448 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610448 Rev.1
      报价单和参考
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      研发参考 (5)

      1. DeMali KA, Barlow CA, Burridge K. Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion. J Cell Biol. 2002; 159(5):881-891. (Clone-specific: Western blot). 查看参考
      2. Haffner C, Jarchau T, Reinhard M, Hoppe J, Lohmann SM, Walter U. Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP. EMBO J. 1995; 14(1):19-27. (Biology). 查看参考
      3. Howe AK, Hogan BP, Juliano RL. Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion. J Biol Chem. 2002; 277(41):38121-38126. (Clone-specific: Western blot). 查看参考
      4. Lawrence DW, Pryzwansky KB. The vasodilator-stimulated phosphoprotein is regulated by cyclic GMP-dependent protein kinase during neutrophil spreading. J Immunol. 2001; 166(9):5550-5556. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
      5. Reinhard M, Giehl K, Abel K. The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. EMBO J. 1995; 14(8):1583-1589. (Biology). 查看参考
      查看所有文件 (5) 查看更少内容
      610448 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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