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Western blot analysis of RONα on a MDCK cell lysate (Canine kidney; ATCC CCL-34). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RONα antibody.
Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma; ATCC HTB-134).
BD Transduction Laboratories™ Purified Mouse Anti-RONα
BD Transduction Laboratories™ Purified Mouse Anti-RONα
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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RON (Recepteur d'Origine Nantaise) is a tyrosine kinase receptor for MSP (Macrophage Stimulating Protein) that belongs to the hepatocyte growth factor receptor family. RON is synthesized as a single chain glycosylated polypeptide of 1400 amino acids that is cleaved upon maturation into a 150 kDa β-subunit with the kinase activity and a 40 kDa α-chain. Both chains are linked by disulfide bonds and the proper arrangement of the α and β heterodimer is necessary for biological activity of RON. MSP induces the tyrosine kinase activity of RON, triggering a cascade of intracellular signaling that leads to DNA synthesis. However, RON activation in mouse erythroleukemia cells results in apoptosis. The replacement of autophosphorylation sites Y1330 and Y1337 abolishes both the mitogenic and apoptotic effects of MSP. Therefore, autophosphorylation at Y1330 and Y1337 is required for RON's biological activity. PLCγ, Shc, Grb2, p61, and p65 associate with RON. Although widely expressed, RON is particularly abundant in epithelial tissues, lung, brain, adrenal glands, gastrointestinal tract, kidney, and testis.
研发参考 (4)
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Gaudino G, Follenzi A, Naldini L. RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP. EMBO J. 1994; 13(15):3524-3532. (Biology). 查看参考
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Peace BE, Hughes MJ, Degen SJ, Waltz SE. Point mutations and overexpression of Ron induce transformation, tumor formation, and metastasis. Oncogene. 2001; 20(43):6142-6151. (Biology: Immunoprecipitation, Western blot). 查看参考
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Ronsin C, Muscatelli F, Mattei MG, Breathnach R. A novel putative receptor protein tyrosine kinase of the met family. Oncogene. 1993; 8(5):1195-11202. (Biology). 查看参考
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Wang MH, Ronsin C, Gesnel MC, Coupey L. Identification of the ron gene product as the receptor for the human macrophage stimulating protein. Science. 1994; 266(5182):117-119. (Biology). 查看参考
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