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      Purified Mouse Anti-Rat TGN38
      Purified Mouse Anti-Rat TGN38
      Western blot analysis of TGN38 on a rat cerebum lysate.  Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- rat TGN38 antibody.
      Purified Mouse Anti-Rat TGN38
      Immunofluorescence staining of L6 cells (Rat skeletal muscle myoblasts; ATCC CRL-1458).
      Purified Mouse Anti-Rat TGN38 Purified Mouse Anti-Rat TGN38
      Western blot analysis of TGN38 on a rat cerebum lysate.  Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- rat TGN38 antibody.
      Immunofluorescence staining of L6 cells (Rat skeletal muscle myoblasts; ATCC CRL-1458).
      商品详情
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      BD Transduction Laboratories™
      Trans Golgi Network-38
      Rat (QC Testing)
      Mouse IgG1
      Rat TGN38 aa. 31-244
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
      85-95 kDa
      250 µg/ml
      AB_398216
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      610898 Rev. 1
      抗体详情
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      2/TGN38

      Newly synthesized proteins exit the ER and move to the cis-Golgi network (CGN) where they traverse the cis-medial and trans-cisternae before reaching the trans-Golgi network (TGN). N-linked oligosaccharide processing occurs in the TGN, and proteins are sorted to the plasma membrane, lysosomes, endosomes, and secretory granules. TGN38 is a type I integral membrane protein primarily localized to the TGN. It is involved in the sorting of nascent proteins into  individual carrier vesicles for transport to appropriate destinations. It is thought to heterodimerize with TGN41 and participate in exocytic budding from the TGN. TGN38 has a molecular weight of 85 to 95 kDa. The core polypeptide represents approximately 38 kDa, while the remainder is accounted for by N- and O-linked oligosaccharide chains. A 286 aa N-terminal lumenal domain, a 21 aa membrane spanning domain, and a 33 aa C-terminal cytoplasmic tail comprise the structure of TGN38. The cytoplasmic tail contains a tyrosine-based motif, YQRL, that is thought to be involved in TGN localization. Therefore, TGN38 mediates the localization of various proteins to the TGN and serves as a TGN retrieval signal.

      This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      610898 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610898 Rev.1
      报价单和参考
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      研发参考 (5)

      1. Humphrey JS, Peters PJ, Yuan LC, Bonifacino JS. Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence. J Cell Biol. 1993; 120(5):1123-1135. (Biology). 查看参考
      2. Luzio JP, Brake B, Banting G, Howell KE, Braghetta P, Stanley KK. Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38). Biochem J. 1990; 270(1):97-102. (Biology). 查看参考
      3. Mary S, Charrasse S, Meriane M, et al. Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism. Mol Biol Cell. 2002; 13(1):285-301. (Biology: Immunofluorescence). 查看参考
      4. Ozawa K, Kondo T, Hori O. Expression of the oxygen-regulated protein ORP150 accelerates wound healing by modulating intracellular VEGF transport. J Clin Invest. 2001; 108(1):39-40. (Biology: Western blot). 查看参考
      5. Xu L, Shen Y, Joseph T, et al. Mitogenic phospholipase D activity is restricted to caveolin-enriched membrane microdomains. Biochem Biophys Res Commun. 2000; 273(1):77-83. (Biology: Western blot). 查看参考
      查看所有文件 (5) 查看更少内容
      610898 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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