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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, like many of its relatives, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. In addition, IQGAP1 has been identified as a GTPase activating protein specific for Rac1. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. BD Biosciences, however, has not performed such cross-reactivity testing.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Didsbury J, Weber RF, Bokoch GM, Evans T, Snyderman R. rac, a novel ras-related family of proteins that are botulinum toxin substrates. J Biol Chem. 1989; 264(28):16378-16382. (Biology). 查看参考
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Doye A, Mettouchi A, Bossis G, et al. CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion. Cell. 2002; 111(4):553-564. (Clone-specific: Immunofluorescence, Western blot). 查看参考
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Habas R, Dawid IB, He X. Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation. Genes Dev. 2003; 17(2):295-309. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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Innocenti M, Tenca P, Frittoli E, et al. Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002; 156(1):125-136. (Clone-specific: Immunofluorescence, Western blot). 查看参考
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Nobes CD, Hall A. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell. 1995; 81(1):53-62. (Biology). 查看参考
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