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Cell Preparation and Separation Reagents
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- BD® Single-Cell Multiplexing Kit
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- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Western blot analysis of rabaptin-5 on a MCF-7 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- rabaptin-5 antibody.
Immunofluorescence staining of A431 cells.
BD Transduction Laboratories™ Purified Mouse Anti-Rabaptin-5
BD Transduction Laboratories™ Purified Mouse Anti-Rabaptin-5
监管状态图例
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Rabs are small GTP-binding proteins that are localized to specific intracellular vesicles and organelles. They are important for the regulation of vesicular traffic. It is thought that Rabs cycle between GTP- and GDP-bound forms and this cycle is related to their function. Rabaptin-5 is protein that interacts with GTP-Rab5. Although Rabaptin-5 is localized in the cytosol, it is also recruited to the endosomal fraction in a Rab5 and GTP-dependent manner. The removal of Rabaptin-5 from the cytosol substantially impairs GTP and Rab5-dependent endosomal fusion. This indicates that Rabaptin-5 may be an important partner of Rab5 during endocytosis. Rabaptin-5 contains 862 amino acids and both ends of the protein contain coiled-coil domains typically found in vesicular transport proteins.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (3)
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Shiba Y, Takatsu H, Shin HW, Nakayama K. Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain. J Biochem (Tokyo). 2002; 131(3):327-336. (Biology: Western blot). 查看参考
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Stenmark H, Vitale G, Ullrich O, Zerial M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995; 83(3):423-432. (Biology). 查看参考
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Zhu Y, Doray B, Poussu A, Lehto VP, Kornfeld S. Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor. Science. 2001; 292(5522):1716-1718. (Biology: Immunofluorescence, Immunoprecipitation). 查看参考
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