BD Transduction Laboratories™ Purified Mouse Anti-PTP1B
克隆 15/PTP1B (RUO)
Western blot analysis of PTP1B on SW13 lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of PTP1B.
HCT-8
Western blot analysis of PTP1B on SW13 lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of PTP1B.
HCT-8
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
PTP1B is a member of the class of enzymes termed protein tyrosine phosphatases (PTPs). This 50 kDa protein contains a conserved phosphatases domain at residues 30-278 and is localized to the cytoplasmic face of the endoplasmic reticulum by its C-terminal 35 residues. Its interactions with other proteins are mediated by proline-rich regions and SH2 compatible sequences. PTP1B is thought to act as a negative regulator in insulin signaling. Insulin stimulation induces its association with the insulin receptor and tyrosine phosphorylation. Its overexpression inhibits proximal and distal insulin signaling events, possibly due to receptor dephosphorylation. Microinjection of PTP1B into Xenopus oocytes blocks insulin-induced maturation. Additional studies demonstrate that PTP1B undergoes cell cycle-dependent and/or stress-induced serine phosphorylation. This suggests further regulatory roles that are not yet defined.
研发参考 (5)
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Bharadwaj S, Ali A, Ovsenek N. Multiple components of the HSP90 chaperone complex function in regulation of heat shock factor 1 In vivo. Mol Biol Cell. 1999; 19(12):8033-8041. (Biology). 查看参考
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Gaits F, Li RY, Bigay J, Ragab A, Ragab-Thomas MF, Chap H. G-protein beta gamma subunits mediate specific phosphorylation of the protein-tyrosine phosphatase SH-PTP1 induced by lysophosphatidic acid. J Biol Chem. 1996; 271(33):20151-20155. (Biology). 查看参考
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Rutledge TW, Whiteheart SW. SNAP-23 is a target for calpain cleavage in activated platelets. J Biol Chem. 2002; 277(40):37009-37015. (Clone-specific: Western blot). 查看参考
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Tao J, Malbon CC, Wang HY. Galpha(i2) enhances insulin signaling via suppression of protein-tyrosine phosphatase 1B. J Biol Chem. 2001; 276(43):39705-39712. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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Xu G, Arregui C, Lilien J, Balsamo J. PTP1B modulates the association of beta-catenin with N-cadherin through binding to an adjacent and partially overlapping target site. J Biol Chem. 2002; 277(51):49989-49997. (Clone-specific: ELISA, Western blot). 查看参考
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