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Immunofluorescence. Top row: Anti-Phosphoserine (Cat. No. 612546). Bottom Row: Phosphoserine/threonine (Cat. No. 612548). Left panels: untreated A431 cells control, Right panels: A431 & CalyculinA/ Okadaic Acid (2 hour treatment).
BD Transduction Laboratories™ Purified Mouse Anti-Phosphoserine
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
Western blot: Typically, 1:2500 is a useful dilution for use in Western Blot. For specific procedures, please refer to our web site at http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.
研发参考 (3)
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Brivanlou AH, Darnell JE Jr. Signal transduction and the control of gene expression. Science. 2002; 295(5556):813-818. (Biology). 查看参考
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Pawson T, Scott JD. Signaling through scaffold, anchoring, and adaptor proteins. Science. 1997; 278(5346):2075-2080. (Biology). 查看参考
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Yan JX, Packer NH, Gooley AA, Williams KL. Protein phosphorylation: technologies for the identification of phosphoamino acids. J Chromatogr A. 1998; 808(1-2):23-41. (Biology). 查看参考
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