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      Purified Mouse Anti-nNOS
      Purified Mouse Anti-nNOS
      Purified Mouse Anti-nNOS
      Purified Mouse Anti-nNOS
      Purified Mouse Anti-nNOS

      Western blot analysis of nNOS on a rat pituitary lysate.  Lane 1: 1:2500, lane 2: 1: 5000, lane 3: 1: 10,000 dilution of the mouse anti-nNOS antibody.

      610308
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      ¥4,428.00
      EA (1 Each)
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      保存至购物清单请求散装试剂报价
      商品详情
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      BD Transduction Laboratories™
      NOS Type I; Neuronal Nitric Oxide Synthase
      Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
      Mouse IgG2a
      Human nNOS aa. 1095-1289
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
      155 kDa
      250 µg/ml
      AB_397700
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      数据表

      Download技术数据表（TDS）Download安全数据表
      610308 Rev. 1
      抗体详情
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      16/nNOS/NOS Type I

      Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO).  NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity.  In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding.  Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner.  However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity.  Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.  Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum.  The NO synthesized by this enzyme acts as a neurotransmitter.  ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization.

      This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.

      610308 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610308 Rev.1
      报价单和参考
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      研发参考 (5)

      1. Bredt DS, Hwang PM, Glatt CE, Lowenstein C, Reed RR, Snyder SH. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature. 1991; 351(6329):714. (Biology). 查看参考
      2. Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992; 6(12):3051-3064. (Biology). 查看参考
      3. Sasaki M, Gonzalez-Zulueta M, Huang H. Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism. Proc Natl Acad Sci U S A. 2000; 97(15):8617-8622. (Biology: Immunofluorescence, Western blot). 查看参考
      4. Schuh K, Uldrijan S, Telkamp M, Rothlein N, Neyses L. The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I. J Cell Biol. 2001; 155(2):201-205. (Biology: Immunoprecipitation, Western blot). 查看参考
      5. Yu Q, Shao R, Qian HS, George SE, Rockey DC. Gene transfer of the neuronal NO synthase isoform to cirrhotic rat liver ameliorates portal hypertension. J Clin Invest. 2000; 105(6):741-748. (Biology: Immunohistochemistry, Western blot). 查看参考
      查看所有文件 (5) 查看更少内容
      610308 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.